Near-field scanning optical microscopy detects nanoscale glycolipid domains in the plasma membrane

被引：9

作者：

Abulrob, A.

Lu, Z. ^{[1
]}

Brunette, E.

Pulla, D. ^{[1
]}

Stanimirovic, D.

Johnston, L. J. ^{[1
]}

机构：

[1] Natl Res Council Canada, Steacie Inst Mol Sci, Ottawa, ON K1A 0R6, Canada

来源：

JOURNAL OF MICROSCOPY | 2008年 / 232卷 / 02期

关键词：

Confocal microscopy; glycolipid; membrane rafts; near-field scanning optical microscopy;

D O I：

10.1111/j.1365-2818.2008.02093.x

中图分类号：

TH742 [显微镜];

学科分类号：

摘要：

The localization of asialo-GM1 in ordered membrane raft domains in HeLa cells has been examined using a combination of membrane fractionation and fluorescence imaging. The glycolipid is enriched in Triton X-100 insoluble membrane fractions that contain high concentrations of cholesterol and caveolin-1 but is also found in detergent soluble membrane fractions. Near-field fluorescence microscopy shows that a fraction of the asialo-GM1 is localized in small nanoscale clusters that have an upper limit for the average diameter of approximately 90 nm and are partially colocalized with caveolae membrane domains. In addition to clusters, a diffuse, non-clustered population of asialo-GM1 is observed and is hypothesized to correspond to glycolipid isolated in detergent soluble membrane fractions.

引用

收藏

页码：225 / 234

页数：10

相关论文

共 36 条

[1] The blood-brain barrier transmigrating single domain antibody: mechanisms of transport and antigenic epitopes in human brain endothelial cells [J].

Abulrob, A ;

Sprong, H ;

Henegouwen, PVBE ;

Stanimirovic, D .

JOURNAL OF NEUROCHEMISTRY, 2005, 95 (04) :1201-1214

[2] Protection by cholesterol-extracting cyclodextrins:: a role for N-methyl-D-aspartate receptor redistribution [J].

Abulrob, A ;

Tauskela, JS ;

Mealing, G ;

Brunette, E ;

Faid, K ;

Stanimirovic, D .

JOURNAL OF NEUROCHEMISTRY, 2005, 92 (06) :1477-1486

[3] Interactions of EGFR and caveolin-1 in human glioblastoma cells: evidence that tyrosine phosphorylation regulates EGFR association with caveolae [J].

Abulrob, A ;

Giuseppin, S ;

Andrade, MF ;

McDermid, A ;

Moreno, M ;

Stanimirovic, D .

ONCOGENE, 2004, 23 (41) :6967-6979

[4] Imaging intracellular fluorescent proteins at nanometer resolution [J].

Betzig, Eric ;

Patterson, George H. ;

Sougrat, Rachid ;

Lindwasser, O. Wolf ;

Olenych, Scott ;

Bonifacino, Juan S. ;

Davidson, Michael W. ;

Lippincott-Schwartz, Jennifer ;

Hess, Harald F. .

SCIENCE, 2006, 313 (5793) :1642-1645

[5] A fast, simple and sensitive method for the detection and quantification of detergent-resistant membranes [J].

Blank, N ;

Gabler, C ;

Schiller, M ;

Kriegel, M ;

Kalden, JR ;

Lorenz, HM .

JOURNAL OF IMMUNOLOGICAL METHODS, 2002, 271 (1-2) :25-35

[6] Structure and function of sphingolipid- and cholesterol-rich membrane rafts [J].

Brown, DA ;

London, E .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2000, 275 (23) :17221-17224

[7] Near-field scanning optical microscopy probes: a comparison of pulled and double-etched bent NSOM probes for fluorescence imaging of biological samples [J].

Burgos, P ;

Lu, Z ;

Ianoul, A ;

Hnatovsky, C ;

Viriot, ML ;

Johnston, LJ ;

Taylor, RS .

JOURNAL OF MICROSCOPY, 2003, 211 :37-47

[8] Nanoscale organization of the pathogen receptor DC-SIGN mapped by single-molecule high-resolution fluorescence microscopy [J].

de Bakker, Baerbel I. ;

de lange, Frank ;

Cambi, Alessandra ;

Korterik, Jeroen P. ;

van Dijk, Erik M. H. P. ;

van Hulst, Niek F. ;

Figdor, Carl G. ;

Garcia-Parajo, Maria F. .

CHEMPHYSCHEM, 2007, 8 (10) :1473-1480

[9] Macromolecular-scale resolution in biological fluorescence microscopy [J].

Donnert, Gerald ;

Keller, Jan ;

Medda, Rebecca ;

Andrei, M. Alexandra ;

Rizzoli, Silvio O. ;

Luehrmann, Reinhard ;

Jahn, Reinhard ;

Eggeling, Christian ;

Hell, Stefan W. .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2006, 103 (31) :11440-11445

[10] Near-field scanning optical microscopy [J].

Dunn, RC .

CHEMICAL REVIEWS, 1999, 99 (10) :2891-+

← 1 2 3 4 →