肌肉注射人神经生长因子基因逆轴突传递修复神经损伤的研究进展

被引：3

作者：

李红星 ^{[1
]}

彭肖肖 ^{[2
]}

申汉威 ^{[1
]}

李俊卿 ^{[1
]}

杨孔宾 ^{[1
]}

机构：

[1] 哈尔滨医科大学附属第一医院神经外科

[2] 东营市人民医院(山东省立医院集团东营医院)重症医学科

来源：

现代生物医学进展 | 2015年 / 15卷 / 04期

关键词：

神经生长因子; 基因治疗; 肌肉注射; 逆轴突传递; 神经损伤;

D O I：

10.13241/j.cnki.pmb.2015.04.049

中图分类号：

R741 [神经病学];

学科分类号：

1002 ;

摘要：

神经生长因子(NGF)促进中枢及外周神经系统神经元细胞存活、分化、轴突再生等重要作用已得到临床的广泛证实。目前临床上主要以局部或肌肉注射NGF蛋白的方式对神经系统的损伤进行治疗。但NGF半衰期短、局部应用副作用大、费用昂贵、难以透过血脑屏障等缺点而限制临床应用。长期以来,科研工作者致力于寻求一种理想的途径或方法以克服这一缺陷。随着基因工程技术的飞速发展,研究人员发现通过骨骼肌肌肉注射途径,以非病毒载体介导外源的NGF基因体内表达并逆轴突传递到神经损伤部位,有望解决这一难题。本文将就NGF及受体的基本结构和特性、逆轴突传递的机制、非病毒载体结合骨骼肌肌肉注射的基因治疗等方面进行总结和阐述。

引用

页码：788 / 791+795 +795

页数：5

共 16 条

[1]

Nerve growth factor reduces myocardial ischemia/reperfusion injury in rat hearts[J] . Jennifer L. Strande,Kasi V. Routhu,Shimon Lecht,Philip Lazarovici.Journal of Basic and Clinical Physiology and Pharmacology . 2013 (1)

[2] Long-Term Follow-Up Evaluation of Results From Clinical Trial Using Hepatocyte Growth Factor Gene to Treat Severe Peripheral Arterial Disease [J].

Makino, Hirofumi ;

Aoki, Motokuni ;

Hashiya, Naotaka ;

Yamasaki, Keita ;

Azuma, Junya ;

Sawa, Yoshiki ;

Kaneda, Yasufumi ;

Ogihara, Toshio ;

Morishita, Ryuichi .

ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY, 2012, 32 (10) :2503-+

[3]

Neurotrophin Signaling via Long-Distance Axonal Transport[J] . Praveen D. Chowdary,Dung L. Che,Bianxiao Cui.Annual Review of Physical Chemistry . 2012

[4] Recombinant human nerve growth factor (rhNGF-β) gene transfer promotes regeneration of crush-injured mental nerve in rats [J].

Li, Bo-Han ;

Kim, Soung-Min ;

Yoo, Sang Bae ;

Kim, Myung-Jin ;

Jahng, Jeong Won ;

Lee, Jong-Ho .

ORAL SURGERY ORAL MEDICINE ORAL PATHOLOGY ORAL RADIOLOGY, 2012, 113 (03) :E26-E34

[5]

Liposome–polyethylenimine complexes for enhanced DNA and siRNA delivery[J] . Biomaterials . 2010 (26)

[6] Nerve Growth Factor in Rheumatic Diseases [J].

Seidel, Matthias F. ;

Herguijuela, Marjeta ;

Forkert, Randolf ;

Otten, Uwe .

SEMINARS IN ARTHRITIS AND RHEUMATISM, 2010, 40 (02) :109-126

[7]

Targeted nonviral delivery vehicles to neural progenitor cells in the mouse subventricular zone[J] . Ester J. Kwon,Jurate Lasiene,Berit E. Jacobson,In-Kyu Park,Philip J. Horner,Suzie H. Pun.Biomaterials . 2009 (8)

[8]

A Functional Dynein–Microtubule Network Is Required for NGF Signaling Through the Rap1/MAPK Pathway[J] . ChengbiaoWu,AlfredoRamirez,BianxiaoCui,JianqingDing,Jean‐Dominique M.Delcroix,Janice S.Valletta,Jia‐JiaLiu,YanminYang,StevenChu,William C.Mobley.Traffic . 2007 (11)

[9] Neuron-specific delivery of nucleic acids mediated by Tet1-modified poly(ethylenimine) [J].