补阳还五汤对大鼠局灶性脑缺血后血管内皮生长因子及其受体Flk1的影响

被引：45

作者：

刘柏炎 ^{[1
]}

蔡光先 ^{[1
]}

刘维 ^{[2
]}

陈雪梅 ^{[1
]}

机构：

[1] 湖南中医药大学内科实验室

[2] 中南大学湘雅医院

来源：

中草药 | 2007年 / 03期

关键词：

补阳还五汤; 局灶性脑缺血; 血管内皮生长因子(VEGF); 血管内皮生长因子受体(Flk1);

D O I：

暂无

中图分类号：

R285.5 [中药实验药理];

学科分类号：

1008 ;

摘要：

目的探讨补阳还五汤对大鼠局灶性脑缺血后血管内皮生长因子(VEGF)及其受体(Fetal liver kinase1,Flk1)的影响。方法大鼠随机分为假手术组、模型组、尼莫地平组、补阳还五汤组,大脑中动脉线栓法复制局灶性脑缺血模型,采用免疫组织化学法和酶联免疫法检测各组动物脑内VEGF、Flk1的表达和蛋白水平,同时评价动物神经功能。结果在正常大鼠脑内有少量VEGF和Flk1阳性细胞,脑缺血后VEGF和Flk1阳性细胞增加;与模型组比较,尼莫地平、补阳还五汤均能改善模型大鼠的神经功能缺失症状,增强VEGF和Flk1的表达,提高VEGF蛋白水平(P<0.05);于7、14d补阳还五汤作用强于尼莫地平(P<0.05)。结论补阳还五汤增强大鼠局灶性脑缺血后VEGF及Flk1的表达和提高VEGF蛋白水平,是其抗脑缺血的可能作用机制之一。

引用

页码：394 / 397

页数：4

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