重组人促红细胞生成素对急性脑损伤大鼠的神经保护作用研究

被引：2

作者：

于代华

孙绪德

柴伟

高昌俊

机构：

[1] 第四军医大学唐都医院麻醉科

来源：

重庆医学 | 2015年 / 44卷 / 18期

关键词：

红细胞生成素; 脑损伤; 谷氨酸转运体1; 天冬氨酸氨基转移酶类;

D O I：

暂无

中图分类号：

R651.15 [];

学科分类号：

1002 ; 100210 ;

摘要：

目的探讨重组人促红细胞生成素(rh-EPO)对大鼠急性脑损伤后脑细胞凋亡的保护作用及对GLT-1/GLAST表达的影响。方法 60只大鼠分为对照组(n=18)、急性脑损伤组(n=22)和rh-EPO处理组(n=20),急性脑损伤组用改良Feeney′s自由落体打击器制作急性脑损伤模型,rh-EPO处理组采用同样的方法制作脑外伤模型15min后腹腔注射rh-EPO,所有动物在实验完成48h后断头取脑。应用RT-PCR、Western blot检测GLT-1、GLAST mRNA及蛋白表达。结果急性脑损伤48h后,大鼠缺血区GLT-1和GLAST mRNA及蛋白表达较对照组均显著降低(均P<0.01),而rh-EPO处理组GLT-1和GLAST的mRNA及蛋白表达较急性脑损伤组均显著增加(均P<0.01)。结论 EPO可显著减轻大鼠急性脑损伤,其机制可能与GLT-1/GLAST表达上调有关。

引用

页码：2474 / 2476

页数：3

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