过表达Fibulin-5对大鼠脑缺血再灌注损伤后血脑屏障的保护作用

被引：6

作者：

程创

秦新月

郭佳

燕伟平

徐广会

白永生

机构：

[1] 重庆医科大学附属第一医院神经内科,重庆市神经病学重点实验室

来源：

第三军医大学学报 | 2014年 / 36卷 / 13期

关键词：

Fibulin-5; 脑缺血再灌注; 血脑屏障; occludin;

D O I：

10.16016/j.1000-5404.2014.13.022

中图分类号：

R743 [脑血管疾病];

学科分类号：

1002 ;

摘要：

目的探讨过表达Fibulin-5对大鼠脑缺血再灌注损伤后血脑屏障的保护作用及其可能的机制。方法将112只成年雄性SD大鼠分成4组:假手术组(S)、脑缺血组(I/R)、脑缺血加过表达Fibulin-5腺病毒载体干预组(I/R+AdFBLN)以及脑缺血加空腺病毒载体干预组(I/R+Ad-HK)。大鼠在大脑中动脉阻塞前7 d接受腺病毒注射,再灌注后24 h,采用Western blot、RT-PCR和免疫荧光检测各组大鼠脑组织Fibulin-5和紧密连接蛋白occludin的表达;伊文思蓝漏出评估血脑屏障的通透性;TTC染色检测脑梗死体积并进行神经功能缺损评分。结果脑缺血再灌注24 h,与S组比较,I/R组大鼠伊文思蓝明显漏出(P<0.01),伴有紧密连接蛋白occludin表达降低(P<0.01)。I/R+Ad-FBLN组Fibulin-5的mRNA水平和蛋白水平明显高于其他3组(P<0.01),伊文思蓝漏出明显减少(P<0.01),occludin的表达明显增高(P<0.01),TTC梗死灶明显缩小(P<0.01),神经功能缺损明显减轻(P<0.01)。而I/R+Ad-HK组与I/R组之间差异均无统计学意义(P>0.05)。结论过表达Fibulin-5减轻大鼠脑缺血再灌注损伤后血脑屏障的损害,Fibulin-5促进紧密连接蛋白occludin的表达可能是其保护作用的机制之一。

引用

页码：1386 / 1389

页数：4

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