抑制GSK-3β减轻大鼠急性心肌缺血/再灌注损伤的作用

被引：4

作者：

殷忠 ^{[1
]}

薛白 ^{[2
]}

高好考 ^{[3
]}

王海昌 ^{[3
]}

杨晔 ^{[1
]}

机构：

[1] 海军总医院心内科

[2] 海军总医院消化科

[3] 第四军医大学西京医院心内科

来源：

心脏杂志 | 2011年 / 23卷 / 05期

关键词：

糖原合酶激酶-3β; 缺血/再灌注损伤; 炎症; 核因子-κB; 大鼠;

D O I：

10.13191/j.chj.2011.05.35.yinzh.043

中图分类号：

R965 [实验药理学];

学科分类号：

100602 ; 100706 ;

摘要：

目的:评估糖原合酶激酶-3β(GSK-3β)抑制剂TDZD-8减轻大鼠急性心肌缺血/再灌注损伤(MIRI)的作用,并探讨此作用是否与其下调NF-κB、抑制炎症有关。方法:取健康雄性SD大鼠60只,随机分为缺血/再灌注(I/R)组、I/R+TDZD组、I/R+载体(Vehicle,V)组及假手术(Sham)组。大鼠局部心肌缺血30 min,再灌注3 h。用TTC染色计算心肌梗死面积,HE染色及ELISA法评估心肌组织中中性粒细胞浸润及炎性因子(TNF-α和IL-6)的变化;用Western blot测定心肌组织中NF-κB、GSK-3β磷酸化的水平。结果:TDZD-8能明显降低心肌梗死的面积和心肌组织中性粒细胞浸润、抑制NF-κB激活以及心肌源性TNF-α和IL-6的浓度(P<0.01)。结论:GSK-3β抑制剂TDZD-8能够减轻MIRI,其作用可能与其抑制NF-κB的激活及炎症反应有关。

引用

页码：589 / 593

页数：5

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