LC-MS/MS定量测定大鼠和人肝微粒体孵育液中丁苯酞浓度方法的建立

被引：3

作者：

赵芊 ^{[1
]}

李燕 ^{[2
]}

江骥 ^{[1
]}

胡蓓 ^{[1
]}

机构：

[1] 中国医学科学院&中国协和医科大学北京协和医院临床药理研究中心

[2] 中国医学科学院&中国协和医科大学药物研究所新药开发二室

来源：

分析测试学报 | 2007年 / S1期

关键词：

n-Butylphthalide; LC-MS/MS; Quantitative analysis;

D O I：

暂无

中图分类号：

R96 [药理学];

学科分类号：

100602 ; 100706 ;

摘要：

Here we report a sensitive LC-MS/MS method capable of quantifying n-Butylphthalide (NBP) down to 0.5 ng/mL in rat and human hepatic microsome incubating solutions.The method was validated over linear range of 0.5-4 000 ng/mL using diazepam as the internal standard.Compound was simply prepared by protein precipitation method and analyzed on the LC-MS/MS,which was an API-4000 system equipped with ESI interface and a Symmetry C18 column.The between-day and within-day precision and accuracy of quality control samples were ≤9.3% RSD% and ≤3.3% RE%.Sample stability (Stock solution,freeze/thaw,bench-top,short-term and long-term stability) were also investigated.This method has been applied to drug-drug interaction study of NBP in rat and human hepatic microsome incubating solutions.

引用

页码：100 / 103

页数：4