脑缺血再灌注后大鼠海马CA1区p-JNK表达和Bax的相关性的试验研究

被引：3

作者：

杨倩

周桃峰

吴家幂

机构：

[1] 皖南医学院附属弋矶山医院神经内科

来源：

放射免疫学杂志 | 2007年 / 02期

关键词：

脑缺血-再灌注损伤; C-jun氨基末端激酶; 凋亡蛋白Bax; 细胞凋亡;

D O I：

暂无

中图分类号：

R743 [脑血管疾病];

学科分类号：

1002 ;

摘要：

目的:观察大鼠脑缺血再灌注后海马CA1区活化的JNK蛋白的表达情况,探讨缺血性脑损伤后神经细胞凋亡相关机理。方法:参照ZeaLonga报道的线栓法制备缺血再灌注动物模型,随机将雄性SD大鼠分为正常对照组、缺血再灌注组及假手术对照组。并分别在不同时间点断头取脑制作标本,连续切片作p-JNK、Bax免疫组化染色及HE染色。结果:脑缺血再灌注后海马CA1区p-JNK表达与Bax的表达呈正相关(r=0.838,P<0.01)。p-JNK明显表达时间(2h)略早于Bax的表达,且产生的高峰时间亦稍早。结论:海马CA1区p-JNK表达与Bax的表达呈正相关。

引用

页码：151 / 153

页数：3

共 9 条

[1]

Apaf-1oligomerizes into bio-logically active approximately700-kDa and inactive approximately1.4-MDa apoptosome complexes. Cain K,Braton SB,Langlais C,et al. Journal of Biological Chemistry . 2000

[2]

A critical role of neural-specific JNK3for ischemic apoptosis. Kuan CY,Whitmarsh AJ,Yang DD,et al. Proceedings of the National Academy of Sciences of the United States of America . 2003

[3]

Signal transduction by the JNK group of MAP kinases. Davis RJ. Cell . 2000

[4]

Activa-tion of mitogen-activated protein kinases after transient forebrain ische-mia in gerbil hippocampus. Toshiyuki Sugino,Kazuhiko Nozaki,Yasushi Takagi,et al. The Journal of Nutrition . 2000

[5]

Mitogen-activated protein kinase:Conservation of a three-kinase module from yeast to human. Widmann C,Gibson S,Jarpe MB,et al. Physiological Reviews . 1999

[6]

Mitogen-activated protein kinase:Conservation of a three-kinase module from yeast to human. Widmann C,Gibson S,Jarpe MB,et al. Physiological Reviews . 1999

[7]

The c-jun N-terminal protein kinase signaling pathway mediates Bax activation and subsequent neuronal apoptosis through interaction with bim after transient focal cere-bral ischemia. Okuno S,Saito A,Hayashi T,Chan PH. Neuroscience . 2004

[8]

Reversible middle cereb-ral artery occlusion without craniectomy in rats. Longa EZ.Weinstein PR,Carlson S,et al. Stroke . 1989

[9]

Activation of mitogen-activated protein kinases in experimental cerebral ischemia. Lennmyr F,Karlsson S,Gerwins P,et al. Acta Neurologica Scandinavica . 2002

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