加味独活寄生合剂缓解小鼠膝骨关节炎过程中肠道菌群的参与机制

被引：12

作者：

易南星 ^{[1
,2
]}

米倚林 ^{[1
,2
]}

许晓彤 ^{[1
,2
]}

李纳平 ^{[1
,2
]}

严可 ^{[1
]}

邝高艳 ^{[1
]}

卢敏 ^{[1
]}

机构：

[1] 湖南中医药大学第一附属医院

[2] 湖南中医药大学

来源：

中国药理学通报 | 2022年 / 38卷 / 04期

基金：

湖南省自然科学基金;

关键词：

膝骨关节炎; 肠道菌群; 加味独活寄生合剂; 16S rRNA; DMM手术; 肠道-关节轴;

D O I：

暂无

中图分类号：

R285.5 [中药实验药理];

学科分类号：

100806 [中药药理学];

摘要：

目的本实验拟通过16S rRNA测序研究加味独活寄生合剂调控肠道菌群治疗膝骨关节炎（knee osteoarthritis, KOA）的潜在机制。方法 8周龄雄性C57小鼠随机分为3组：假手术组、KOA模型组、KOA模型+加味独活寄生合剂组（给药组）,6只/组。手术诱导建立KOA模型，给药组以16.25 mL·kg-1灌胃/天，另两组给予生理盐水，8周后取小鼠膝关节和结肠，制备成石蜡切片后行番红O-固绿、免疫组化染色观察软骨变化，16S rRNA测序观察菌群变化。结果番红O-固绿染色发现，与模型组比较，给药组明显增加了软骨面积、降低了OARSI评分；16S rRNA结果表明，加味独活寄生合剂能调控KOA小鼠肠道菌群丰富度，改善Alpha多样性（Chao1、Simpson）和Beta多样性（PCoA、NMDS）。LefSe分析显示，3组间均存在丰度差异明显的物种，包括厚壁菌门、梭杆菌门、变形菌门等。MetaCyc分析发现，加味独活寄生合剂对脂肪酸、糖和氨基酸等代谢途径均有影响（P<0.05）。结论加味独活寄生合剂能够有效保护关节软骨，延缓KOA病程进展，其机制可能是通过调节肠道菌群结构、保护肠道屏障、减轻炎症反应而实现。

引用

页码：625 / 632

页数：8

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