大鼠尾加压素Ⅱ收缩大鼠离体肺主动脉环与MAPK相关(英文)

被引：6

作者：

陈少贤

薛必成

龚永生

范小芳

胡良刚

陈彦凡

王良兴

机构：

[1] 浙江省温州医学院附属一医呼吸内科,浙江省温州医学院附属一医呼吸内科,浙江省温州医学院肺心病研究室,浙江省温州医学院肺心病研究室,浙江省温州医学院肺心病研究室,浙江省温州医学院附属一医呼吸内科,浙江省温州医学院附属一医呼吸内科浙江温州　,浙江温州,浙江温州　,浙江温州　,浙江温州　,浙江温州　,浙江温州

来源：

中国病理生理杂志 | 2003年 / 10期

关键词：

尿紧张素类; 有丝分裂素激活蛋白激酶类; 肺动脉;

D O I：

暂无

中图分类号：

R363 [病理生理学];

学科分类号：

100104 ;

摘要：

目的 :研究大鼠尾加压素II(U -II)对大鼠离体肺主动脉环的收缩效应及与细胞信号转导通路丝裂素活化蛋白激酶 (MAPK)的关系。方法 :从雄性Sprague -Dauley大鼠中分离出肺主动脉 ,切成 3- 4mm的血管环 ,制备大鼠U -II(0 .0 3- 30nmol/L)的浓度 -效应收缩曲线 ,另外 ,用大鼠U -II(30nmol/L)预收缩血管达平台期后 ,加入MAPK阻断剂PD 980 5 9,制备PD 980 5 9(0 .1μmol/L - 10 μmol/L)浓度 -效应舒张曲线 ,最后分别计算EC50 和Emax。结果 :大鼠U -II是大鼠离体肺主动脉环的有效血管收缩剂 [EC50 =7.95± 0 .4 0 ,Emax=(14 .2 8± 6 .34) % ,以 6 0mmolKCl的收缩幅度为 10 0 % ];PD 980 5 9呈浓度依赖性舒张大鼠U -II预收缩的动脉 [EC50 =5 .91± 0 .4 5 ,Emax=(81.39±13.6 5 ) % ]。结论 :大鼠尾加压素II是大鼠肺主动脉有效的血管收缩剂 ,细胞信号转导通路MAPK的激活参与其收缩效应。

引用

页码：70 / 73

页数：4

共 11 条

[1]

Cloning of the cDNA encoding the urotensin-II precursor in frog and human reveals intense expression of the urotensin-II gene in motoneurons of the spinal cord. Coulouarn Y,Lihrmann I,Jegou S,et al. Proceedings of the National Academy of Sciences of the United States of America . 1998

[2]

Urotensin- II: a somatostatin-like peptide in the caudal neurosecretory system of fishes. Pearson D,Shively JE,Clark BR,et al. Proceedings of the National Academy of Sciences of the United States of America . 1980

[3]

Differential vasoconstrictor activity of human urotensin- II in vascular tissue isolated from the rat, mouse, dog, pig, marmoset and cynomolgus monkey. Douglas SA,Sulpizio AC,Piercy V,et al. British Journal of Pharmacology . 2000

[4]

Molecular and pharmacological characterization of genes encoding urotensin-II peptides and their cognate G-protein-coupled receptors from the mouse and monkey. Elshourbagy NA,Douglas SA,Shabon U,et al. British Journal of Pharmacology . 2002

[5]

Orphan-receptor ligand human urotensin- II: receptor localization in human tissues and comparison of vasoconstrictor responses with endothelin-1. Maguire JJ,Kuc RE,Davenport AP,et al. British Journal of Pharmacology . 2000

[6]

Human urotensin-II-induced aorta ring contractions are mediated by protein kinase C, tyrosine kinases and Rho-kinase: inhibition by somatostatin receptor antagonists. Rossowski WJ,Cheng BL,Taylor JE,et al. European Journal of Pharmacology . 2002

[7]

Involvement of p44/42 mitogen-activated protein kinases in regulating angiotensin II- and endothelin-1- induced contraction of rat thoracic aorta. Ishihata A,Tasaki K,Katano Y,et al. European Journal of Pharmacology . 2002

[8]

Synergistic effect of urotensin- II with serotonin on vascular smooth muscle cell proliferation. Watanabe T,Pakala R,Katagiri T,et al. Journal of Hypertension . 2001

[9]

Contractile responses to human urotensin-II in rat and human pulmonary arteries: effect of endothelial factors and chronic hypoxia in the rat. Maclean M R,Alexander D,Stirrat A,et al. British Journal of Pharmacology . 2000

[10]

Human urotensinII is a potent vasoconstrictor and agonist for the orphan receptor GPR14. JD,Liu WS,Glover GI,Wilson S,et al. Nature . 1999

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