新生大鼠缺血缺氧后脑内一氧化氮合酶的动态表达

被引：4

作者：

岳旭

郭艳茹

机构：

[1] 中日友好医院临床医学研究所

[2] 中日友好医院临床医学研究所北京

[3] 北京

来源：

中国组织化学与细胞化学杂志 | 2001年 / 04期

关键词：

脑缺血缺氧; 一氧化氮合酶; 免疫组织化学; 新生大鼠;

D O I：

暂无

中图分类号：

R363 [病理生理学];

学科分类号：

100104 ;

摘要：

实验采用生后 14天 Wistar大鼠缺血缺氧 (HI)动物模型 ,用免疫组织化学方法观察 HI复苏 (HI/ R)后前脑一氧化氮合酶动态表达。结果显示 :神经元型一氧化氮合酶 (n NOS)阳性神经元主要分布于新生大鼠大脑皮层的 - 层 ,尾状核、隔核及嗅结节。 HI/ R早期其表达水平无明显变化 ;复苏 48小时及 5天后 ,可分别在右侧大脑顶皮层或右侧大脑顶皮层和尾状核区出现梗塞灶 ,该区 n NOS阳性神经元明显减少。而诱导型一氧化氮合酶 (i NOS)阳性细胞在 HI/ R后 12小时始现于损伤侧的侧脑室 ;随时间的推移在损伤侧缰核、皮层、尾状核以及丘脑背外侧核、丘脑腹侧核可见 i NOS阳性细胞逐渐增多并染色加深。用识别单核巨噬细胞的克隆 ED 1单克隆抗体检测可见 ED 1阳性细胞出现的时间和在脑区的分布与 i NOS阳性细胞相似。本实验提示 ,在局灶性脑缺血缺氧早期 ,脑内 NO的释放不依赖于 n NOS阳性神经元或 i NOS阳性细胞 ;而在局灶性脑缺血缺氧晚期 ,i NOS阳性细胞产生的 NO可能参与了脑损伤的过程

引用

页码：429 / 433+478 +478

页数：6

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