cdk-5过度表达对大鼠tau蛋白磷酸化及空间记忆的影响

被引:3
作者
廖晓梅
张迎春
汪义朋
王建枝
机构
[1] 华中科技大学同济医学院病理生理学系,华中科技大学同济医学院病理生理学系,华中科技大学同济医学院病理生理学系,华中科技大学同济医学院病理生理学系武汉,武汉,武汉,武汉
基金
国家杰出青年科学基金;
关键词
细胞周期蛋白依赖性蛋白激酶5; tau蛋白; 空间记忆; Alzheimer病;
D O I
暂无
中图分类号
R749.16 [];
学科分类号
100203 ;
摘要
Alzheimer病(AD)中,异常过度磷酸化的tau蛋白会导致细胞骨架的异常并与神经元的死亡有关.在体外,细胞周期蛋白依赖性蛋白激酶5(cdk-5)能在大多数AD相关位点磷酸化tau蛋白.旨在整体水平研究cdk-5过度表达对大鼠微管相关蛋白tau的磷酸化及空间记忆的影响.结果显示,在大鼠海马区转染 cdk-5基因,24h后其局部表达增加,并使得抗体tau-1显色减弱,PHF-1和12e8显色增强,提示tau蛋白在Ser199/202,Ser396/404和Ser262/356位点过度磷酸化.此外,在水迷宫测试中,cdk-5转染鼠寻找安全平台所需时间比对照鼠明显延长,而转染后48 h cdk-5的表达较 24h时下降,同时伴有tau蛋白磷酸化程度的下降和空间记忆能力的改善.这些结果提示整体水平的cdk-5的过度表达会导致大鼠的空间记忆损伤,而过度磷酸化的tau蛋白可能参与了该病理过程.
引用
收藏
页码:547 / 553
页数:7
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