CHO细胞无血清结团灌流培养:超声-沉降柱二合一灌流系统

被引：2

作者：

李智

肖成祖

杨琴

黄小乐

梁倩茹

陈小飞

郑敦武

陈晓铭

机构：

[1] 广州铭康生物工程有限公司

来源：

中国生物工程杂志 | 2008年 / 04期

关键词：

结团培养; 无血清培养基; CHO细胞; rhTNK-tPA; 超声-沉降柱二合一灌流系统;

D O I：

10.13523/j.cb.20080411

中图分类号：

Q813.1 [细胞、组织培养技术];

学科分类号：

0836 ; 090102 ;

摘要：

利用CHO细胞能在培养过程中自然结团的特性,采用超声—沉降柱二合一灌流系统能促进细胞结团和加强截留的特性,用无血清培养基连续灌流培养基因重组CHO细胞MK3-A2株,分泌表达rhTNK-tPA获得了成功。培养周期为77～110天,细胞结团率为90%左右,直径在285～570μm之间,细胞截留率保持在95%左右,成活率为85%以上,细胞密度达到2×107/ml左右,rhTNK-tPA生产率平均为89mg/L/d,最高时达216mg/L/d。结果表明,使用该灌流系统进行细胞结团培养可以取代微载体培养用于动物细胞制药的规模化生产。

引用

页码：53 / 58

页数：6

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