褪黑素对缺氧缺血性脑损伤新生大鼠的保护作用

被引：5

作者：

乔丽丽 ^{[1
]}

沈伟勤 ^{[1
]}

陈国宏 ^{[1
]}

张小丽 ^{[1
]}

机构：

[1] 上海市松江区中心医院儿科

来源：

实用儿科临床杂志 | 2011年 / 15期

关键词：

褪黑素; 缺氧缺血性脑损伤; 大鼠; 新生;

D O I：

暂无

中图分类号：

R722.1 [新生儿疾病];

学科分类号：

100202 ;

摘要：

目的探讨褪黑素对新生大鼠缺氧缺血性脑损伤(HIBD)的保护作用及其机制。方法 7日龄Wistar大鼠54只结扎左侧颈总动脉,吸入氧氮混合气体55 min制作HIBD模型,随机分成正常对照组、HIBD组和褪黑素治疗组,每组18只。正常对照组既不结扎亦不缺氧,褪黑素治疗组在缺氧缺血前30 min予褪黑素15 mg.kg-1腹腔注射。在缺氧缺血24 h,取每组6只脑组织匀浆用于半胱天冬酶-2、半胱天冬酶-3活性测定,其余每组6只在缺氧缺血72 h取其脑进行石蜡包埋,并进行半胱天冬酶-3、凋亡诱导因子(AIF)及微管相关蛋白-2(MAP-2)的免疫组织化学染色,上述指标用于计算脑梗死体积和海马CA1神经元的丢失,HE染色用于计算脑损伤积分。结果缺氧缺血24 h,HIBD组新生大鼠脑组织半胱天冬酶-2、半胱天冬酶-3活性均明显高于正常对照组(Pa<0.05),给予褪黑素治疗后,半胱天冬酶-2、半胱天冬酶-3活性均明显下降(Pa<0.05);72 h时褪黑素治疗组半胱天冬酶-3和AIF的阳性细胞计数均明显下降(Pa<0.05);褪黑素治疗组脑损伤积分、脑梗死体积、CA1神经元丢失均显著下降(Pa<0.05)。结论褪黑素对HIBD有保护作用,其机制与抑制神经细胞凋亡有关。

引用

页码：1204 / 1206

页数：3

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