大鼠内毒素血症时外周血及肺泡内中性粒细胞死亡方式及呼吸爆发(英文)

被引：1

作者：

李双顶

刘韧

何春阳

肖南

田昆仑

机构：

[1] 西安交通大学医学院,第三军医大学大坪医院野战外科研究所,成都军区总医院理疗科,第三军医大学大坪医院野战外科研究所,第三军医大学大坪医院野战外科研究所西安,重庆,成都,重庆,重庆

来源：

中国实验血液学杂志 | 2002年 / 06期

关键词：

多形核中性粒细胞; 细胞凋亡; 细胞坏死; 呼吸爆发; 内毒素血症;

D O I：

暂无

中图分类号：

R363 [病理生理学];

学科分类号：

100104 ;

摘要：

本研究观察大鼠内毒素血症时肺组织中及外周血多形核中性粒细胞 (PMN)凋亡、坏死及功能改变的差异。采用Wistar大鼠 2 0只 ,腹腔注射LPS(O55B5,5mg/kg)造成内毒素血症 ,给予LPS后 2 ,4,8,1 2小时 (每组 5只动物 )取血及支气管肺泡灌洗 ,密度梯度法分离PMN ,用流式细胞仪测定凋亡和坏死比例以及呼吸爆发功能的改变 ,同时采用 5只大鼠作为正常对照。结果显示 ,内毒素血症时外周血和支气管肺泡灌洗液中PMN凋亡细胞比例相似。但与对照相比 ,外周血PMN坏死比例明显增加 ,呼吸爆发能力明显受抑 ,而支气管肺泡灌洗液PMN坏死比例显著减少 ,呼吸爆发能力显著增强。结论 :在内毒素血症时 ,扣押于肺组织中的PMN在凋亡和坏死上表现出与外周血PMN不同的改变 ,其结果是组织中PMN存活增加 ,并持续处于活化状态 ,这与PMN造成组织损伤有关

引用

页码：503 / 507

页数：5

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