参芎注射液对脑缺血再灌注大鼠炎症因子变化的影响

被引:38
作者
陈孝东
刘春风
曹勇军
王引明
陶峥
机构
[1] 苏州大学附属第二医院神经内科
关键词
脑缺血再灌注; 炎症; 参芎注射液; 大鼠;
D O I
暂无
中图分类号
R285.5 [中药实验药理];
学科分类号
1008 ;
摘要
目的观察脑缺血再灌注(I/R)大鼠脑组织核因子-κB(NF-κB)、肿瘤坏死因子-α(TNF-α)、细胞间粘附分子-1(ICAM-1)表达及血清白细胞介素(IL)-1β、IL-6、IL-10质量浓度的变化,并探讨参芎注射液对其影响。方法2006年4月至7月间于苏州大学附属第二医院将54只健康雄性大鼠随机分为生理盐水对照组、川芎嗪组和参芎组。采用线栓法制作大脑中动脉I/R模型,缺血1h时回抽栓线实现再灌注,造模成功后,于再灌注6h、12h及24h3个时间点断头取脑及采血;采用免疫组化法标记脑组织NF-κB、TNF-α及ICAM-1表达;用酶联免疫吸附试验(ELISA)法检测血清IL-1β、IL-6及IL-10质量浓度。结果各组脑组织TNF-α、ICAM-1均随再灌注时间延长而表达增强,但NF-κB于再灌注12h达高峰;与对照组相比,参芎组NF-κB、TNF-α及ICAM-1表达明显减弱(P均<0.05);参芎可降低IL-1β的质量浓度,促使IL-6的峰值提前,提高IL-10的质量浓度(P<0.05或P<0.01)。结论参芎注射液抑制脑I/R炎症损伤的作用优于川芎嗪。
引用
收藏
页码:1017 / 1020
页数:4
相关论文
共 10 条
[1]   参芎注射液对脑缺血再灌注大鼠行为学和血液流变学的影响 [J].
陈孝东 ;
曹勇军 ;
王引明 ;
陶峥 ;
刘春风 .
中国血液流变学杂志, 2006, (01) :43-45+89
[2]  
Interleukin-10modulates neuronal threshold of vulnerability to ischeamic damage. Grilli M,Barbieri I,Basudev H,et al. European Journal of Neuroscience . 2000
[3]  
Effects of Tanshinone I isola-ted from Salvia miltiorrhiza bunge on arachidonic acid metabolism and in vivo inflammatory responses. Kim SY,Moon TC,Chang HW,et al. Physiother Res . 2002
[4]  
Aqueous extract of Salvia milti-orrhoza regulates adhesion molecule expression of tumor necrosis factor[alpha]-induced endothelial cells by blocking activation of nuclear factor[kappa]B. Ding M,Zhao GR,Yuan YJ,et al. Journal of Cardiovascular Pharmacology . 2005
[5]  
Nuclear factor-[kappa B]contributes to infarction after permanent focal ischemia. Nurmi A,Lindsberg PJ,Koistinaho M,et al. Stroke . 2004
[6]  
Cerebral ischemia and reperfusion:the patho-physiologic concept as a basis for clinical therapy. Schaller B,Graf R. Journal of Cerebral Blood Flow and Metabolism . 2004
[7]  
NF kappa B is acti-vated and promotes cell death in focal cerebral ischemia. Schneider A,Martin-Villalba A,Weih F,et al. Nature Medicine . 1999
[8]  
Transcription factor nu-clear factor-kappa B is activated in neurons after focal cerebral is-chemia. Stephenson D,Yin T,Smalstig EB,et al. Journal of Cerebral Blood Flow and Metabolism . 2000
[9]  
Quantitative changes in interleukin in proteins following focal stroke in the rat. Legos JJ,Whitmore RG,Erhardt JA,et al. Neuroscience Letters . 2000
[10]  
Activation of nuclear transcrip-tion factor kappa B(NF-kappa B)is essential for dopamine in-duced apoptosis in PCL2cells. Panet H,Barzilai A,Daily D,et al. Neurochem . 2001