低温对大鼠全脑缺血再灌注时海马内质网应激的影响

被引：3

作者：

高宇

金利

吴淋

蔡慎权

范婧婧

仲浩

段满林

徐建国

机构：

[1] 南京大学医学院附属金陵医院麻醉科

来源：

中华麻醉学杂志 | 2015年 / 09期

关键词：

低温, 人工; 内质网; 应激; 脑; 再灌注损伤;

D O I：

暂无

中图分类号：

R614 [麻醉学];

学科分类号：

100217 ;

摘要：

目的评价低温对大鼠全脑缺血再灌注时海马内质网应激的影响。方法清洁级健康雄性SD大鼠54只, 体重280～320 g, 采用随机数字表法分为3组(n=18):假手术组(S组)、全脑缺血再灌注组(I/R组)和低温组(H组)。采用经食管心脏起搏诱发心跳骤停+心肺复苏制备大鼠全脑缺血再灌注损伤模型。H组再灌注即刻体表降温, 10 min之内将体温降至32～34 ℃, 维持4 h, 其余组体温维持36～37 ℃。于再灌注24 h时行神经功能缺陷评分, 随后处死大鼠取双侧海马, 采用Western blot法检测葡萄糖调节蛋白78(GRP78)和caspase-12的表达。取全脑组织行HE染色, 观察海马CA1区锥体细胞形态结构, 免疫组化染色法计数GRP78和caspase-12的阳性细胞。结果与S组比较, I/R组和H组再灌注24 h时神经功能缺陷评分升高, 海马CA1区正常锥体细胞数减少, GRP78和caspase-12表达上调, 阳性细胞数增多(P<0.05);与I/R组比较, H组再灌注24 h时神经功能缺陷评分降低, 海马CA1区正常锥体细胞数增多, GRP78表达上调, 阳性细胞数增多, caspase-12表达下调, 阳性细胞数减少(P<0.05)。结论低温可通过抑制海马内质网应激减轻大鼠全脑缺血再灌注损伤。

引用

页码：1146 / 1149

页数：4

共 14 条

[1]

Efficacy of Mild Hypothermia for the Treatment of Patients with Cardiac Arrest[J] Gao Yu;Hui Kang-Li;Wang Yu-Jie;Wu Lin;Duan Man-Lin;Xu Jian-Guo;Li De-Xin; 中华医学杂志英文版 2015, 11

[2]

浅低温对全脑缺血再灌注大鼠海马微小RNA 210表达的影响[J] 张露;陈兴东;赵琳;戴琼艳;惠康丽;徐苗苗;段满林;徐建国; 中华麻醉学杂志 2014,

[3]

Hypothermia protects the brain from transient global ischemia/reperfusion by attenuating endoplasmic reticulum response-induced apoptosis through CHOP.[J] Xiaojie Liu;Mingshan Wang;Huailong Chen;Yunliang Guo;Fuguo Ma;Fei Shi;Yanlin Bi;Ying Li PLoS ONE 2018,

[4]

Adiponectin protects rat myocardium against chronic intermittent hypoxia-induced injury via inhibition of endoplasmic reticulum stress.[J] Wenxiao Ding;Xiaofeng Zhang;Hanpeng Huang;Ning Ding;Shijiang Zhang;Sean Z Hutchinson;Xilong Zhang PLoS ONE 2017,

[5]

Endoplasmic reticulum stress in cerebral ischemia[J] Qing Xin;Bingyuan Ji;Baohua Cheng;Chunmei Wang;Haiqing Liu;Xiaoyu Chen;Jing Chen;Bo Bai Neurochemistry International 2014,

[6]

Improved Early Postresuscitation EEG Activity for Animals Treated with Hypothermia Predicted 96 hr Neurological Outcome and Survival in a Rat Model of Cardiac Arrest[J] Bihua Chen;Feng-Qing Song;Lei-Lei Sun;Ling-Yan Lei;Wei-Ni Gan;Meng-Hua Chen;Yongqin Li;Giuseppe Ristagno BioMed Research International 2013,

[7]

CART attenuates endoplasmic reticulum stress response induced by cerebral ischemia and reperfusion through upregulating BDNF synthesis and secretion[J] Bin Qiu;Shengdi Hu;Libing Liu;Man Chen;Lai Wang;Xianwei Zeng;Shigong Zhu Biochemical and Biophysical Research Communications 2013,

[8]

Comparison of the Durations of Mild Therapeutic Hypothermia on Outcome After Cardiopulmonary Resuscitation in the Rat[J] Sen Ye;Yinlun Weng;Shijie Sun;Wei Chen;Xiaobo Wu;Zilong Li;Max Harry Weil;Wanchun Tang Circulation 2012,

[9]

Integrating the mechanisms of apoptosis induced by endoplasmic reticulum stress.[J] Tabas Ira;Ron David Nature cell biology 2011,

[10]

Part 9: Post-Cardiac Arrest Care: 2010 American Heart Association Guidelines for Cardiopulmonary Resuscitation and Emergency Cardiovascular Care[J] Mary Ann Peberdy;Clifton W. Callaway;Robert W. Neumar;Romergryko G. Geocadin;Janice L. Zimmerman;Michael Donnino;Andrea Gabrielli;Scott M. Silvers;Arno L. Zaritsky;Raina Merchant;Terry L. Vanden Hoek;Steven L. Kronick Circulation 2010,

← 1 2 →