体外标记神经干细胞脑内移植治疗大鼠创伤性脑损伤

被引：5

作者：

杨林

赵洪洋

赵甲山

朱贤立

机构：

[1] 华中科技大学同济医学院附属协和医院神经外科

来源：

中华实验外科杂志 | 2006年 / 02期

关键词：

神经干细胞; 脑损伤; 移植; 氧化铁; 磁共振成像;

D O I：

暂无

中图分类号：

R651.15 [];

学科分类号：

1002 ; 100210 ;

摘要：

目的通过不同的方法对神经干细胞(NSCs)进行标记,观察NSCs脑内移植治疗大鼠创伤性脑损伤后的增殖和迁徙情况。方法Feeney氏法制备大鼠创伤性脑损伤模型,绿色荧光蛋白(GFP)和超顺磁氧化铁(SPIO)标记NSCs后立体定向脑内移植。对移植后大鼠的神经系统行为和运动功能进行评估,免疫组织化学染色观察NSCs的存活及分化情况,并用磁共振成像的方法在体观察NSCs的存活和分布。结果免疫组织化学检查显示脑内移植后部分GFP阳性细胞表达神经元特异性标记物β-tubilinⅢ,神经系统行为学评分显示移植组动物明显改善,NSCs脑内移植后3周磁共振成像显示移植区低信号改变。结论移植NSCs可以有效地促进TBI大鼠神经行为功能的恢复,不同的NSCs标记方法能够综合评价细胞移植后的疗效。

引用

页码：209 / 211+259 +259-260

页数：5

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