吴茱萸次碱对大鼠动脉增龄性改变的干预影响

被引：2

作者：

马懿

吴赛珠

龚勋

莫颖莉

机构：

[1] 南方医科大学南方医院心血管内科

来源：

中华老年心脑血管病杂志 | 2014年 / 16卷 / 07期

关键词：

吴茱萸碱; 超氧化物歧化酶; 丙二醛; 谷胱甘肽; 一氧化氮合酶; RNA,信使; 衰老;

D O I：

暂无

中图分类号：

R285.5 [中药实验药理];

学科分类号：

1008 ;

摘要：

目的观察吴茱萸次碱对大鼠动脉增龄性变化的影响,并探讨其作用机制。方法选择Wistar大鼠32只,分为3月龄组、12月龄组、18月龄组和干预组,每组8只。各组检测超氧化物歧化酶(SOD)、丙二醛以及谷胱甘肽(GSH)含量;实时PCR法检测内皮型一氧化氮合酶(eNOS)mRNA表达;Western blot法测eNOS蛋白和磷酸化蛋白表达。结果与12月龄组比较,18月龄组丙二醛水平明显升高,SOD、GSH、eNOS mRNA、磷酸化eNOS和eNOS蛋白表达明显降低(P<0.05);与18月龄组比较,干预组丙二醛[(4.96±0.22)nmol/mg vs(7.26±0.35)nmol/mg]水平明显降低,SOD[(232.07±11.16)U/mg vs(149.88±13.77)U/mg]、GSH[(12.27±1.42)μg/mg vs(5.40±0.79)μg/mg]、eNOS mRNA[(1.39±0.15)vs(0.74±0.18)]、磷酸化eNOS[(204.67±3.07)vs(121.45±2.54)]和eNOS蛋白[(591.44±2.98)vs(469.57±1.79)]表达明显升高(P<0.05);3月龄组与12月龄组上述指标比较,差异无统计学意义(P>0.05)。结论吴茱萸次碱可以提高自然增龄大鼠动脉血管的抗氧化能力,增加eNOS的表达,从而发挥延缓大鼠血管衰老的作用。

引用

页码：743 / 746

页数：4

共 18 条

[1]

Role of oxidative stress in hypoxia preconditioning of cells transplanted to the myocardium: a molecular imaging study. Aly A,Peterson K,Lerman A,Lerman L,Rodriguez-Porcel M. The Journal of cardiovascular surgery . 2011

[2]

Long-term atorvastatin improves age-related endothelial dysfunction by ameliorating oxidative stress and normalizing eNOS/iNOS imbalance in rat aorta[J] . Xun Gong,Yi Ma,Yunjun Ruan,Guohua Fu,Saizhu Wu. &nbspExperimental Gerontology . 2014

[3] Calcitonin gene-related peptide-mediated depressor effect and inhibiting vascular hypertrophy of rutaecarpine in renovascular hypertensive rats [J].

Qin, Xu-Ping ;

Zeng, Si-Yu ;

Li, Dai ;

Chen, Qing-Quan ;

Luo, Dan ;

Zhang, Zhe ;

Hu, Gao-Yun ;

Deng, Han-Wu ;

Li, Yuan-Jian .

JOURNAL OF CARDIOVASCULAR PHARMACOLOGY, 2007, 50 (06) :654-659

[4] Uncoupling of endothelial NO synthase in atherosclerosis and vascular disease [J].

Li, Huige ;

Foerstermann, Ulrich .

CURRENT OPINION IN PHARMACOLOGY, 2013, 13 (02) :161-167

[5]

Roles of Endothelial Nitric Oxide Synthase (eNOS) and Mitochondrial Permeability Transition Pore (MPTP) in Epoxyeicosatrienoic Acid (EET)-induced Cardioprotection Against Infarction in Intact Rat Hearts[J] . Garrett J. Gross,Anna Hsu,Adam W. Pfeiffer,Kasem Nithipatikom. &nbspJournal of Molecular and Cellular Cardiology . 2012

[6]

Effects of Glutathione-depleting Drug Buthionine Sulfoximine and Aging on Activity of Endothelium-derived Relaxing and Contracting Factors in Carotid Artery of Sprague–Dawley Rats[J] . Steven G Denniss,Andrew S Levy,James W E Rush. &nbspJournal of Cardiovascular Pharmacology . 2011 (3)

[7]

Biological Aging Is No Longer an Unsolved Problem[J] . LEONARDHAYFLICK. &nbspAnnals of the New York Academy of Sciences . 2007 (1)

[8]

Anti-inflammatory effects and mechanisms of the ethanol extract of Evodia rutaecarpa and its bioactive components on neutrophils and microglial cells[J] . Han-Chieh Ko,Yea-Hwey Wang,Kuo-Tong Liou,Chi-Ming Chen,Chih-Hsiang Chen,Wen-Yen Wang,Shiou Chang,Yu-Chang Hou,Kuo-Tung Chen,Chieh-Fu Chen,Yuh-Chiang Shen. &nbspEuropean Journal of Pharmacology . 2006 (2)

[9] eNOS gene influences platelet phenotype in acute coronary syndrome patients on dual antiplatelet treatment [J].

Fatini, Cinzia ;

Sticchi, Elena ;

Bolli, Paola ;

Marcucci, Rossella ;

Giusti, Betti ;

Paniccia, Rita ;

Gori, Anna Maria ;

Gensini, Gian Franco ;

Abbate, Rosanna .

PLATELETS, 2009, 20 (08) :548-554

[10]

Emblica officinalis Exerts Antihypertensive Effect in a Rat Model of DOCA-Salt-Induced Hypertension: Role of (p) eNOS, NO and Oxidative Stress. Jagriti Bhatia,Fauzia Tabassum,Ashok Kumar Sharma. CARDIOVASCULAR TOXICOLOGY . 2011

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