XRCC1与脑缺血再灌注DNA单链断裂

被引：3

作者：

方传勤

周华东

高长越

陆松敏

机构：

[1] 第三军医大学附属大坪医院野战外科研究所脑血管二科

[2] 第三军医大学附属大坪医院野战外科研究所脑血管二科重庆

[3] 重庆

来源：

武警医学 | 2004年 / 08期

关键词：

XRCC1; DNA单链断裂; 凋亡; 脑缺血;

D O I：

10.14010/j.cnki.wjyx.2004.08.013

中图分类号：

R743.3 [急性脑血管疾病（中风）];

学科分类号：

1002 ;

摘要：

目的　观察脑缺血再灌注损伤中XRCC1表达、DNA单链断裂、神经元凋亡的变化 ,探索它们的关系。方法　应用线栓法建立大鼠局灶性脑缺血再灌注模型 :免疫组化检测XRCC1蛋白表达 :单细胞凝胶电泳检测细胞DNA单链断裂 :流式细胞检测细胞凋亡。结果　XRCC1在缺血再灌注 10min后即出现降低 ,1h后整个大脑中动脉供血皮质和基底节区XRCC1进一步下降 ,一直持续到 4 8h。单细胞凝胶电泳示缺血再灌注 10min基底节区出现DNA单链断裂 ,12h达到高峰 ,4 8h仍有DNA单链断裂。流式细胞显示缺血再灌注 4h出现大量细胞凋亡 ,2 4h达到高峰 ,4 8h仍保持较高的水平。结论　在脑缺血再灌注中 ,XRCCl早期降低是DNA单链断裂无法修复的机制之一 ;大量DNA单链断裂和细胞凋亡相关。

引用

页码：595 / 598

页数：4

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