Lox-1受体介导ox-LDL促进血管平滑肌细胞MMP-9 mRNA表达

被引:8
作者
魏盟
魏钧伯
蔡迺绳
机构
[1] 上海市第六人民医院心内科,上海复旦大学附属中山医院心内科,上海复旦大学附属中山医院心内科上海,
关键词
动脉粥样硬化; 氧化低密度脂蛋白; 基质金属蛋白酶-9; 凝集素样氧化低密度脂蛋白受体-1;
D O I
暂无
中图分类号
R543 [血管疾病];
学科分类号
1002 ; 100201 ;
摘要
目的 :观察氧化低密度脂蛋白 (ox LDL)对血管平滑肌细胞 (VSMC)基质金属蛋白酶 9(MMP 9)表达的影响及凝集素样氧化低密度脂蛋白受体 1(Lox 1)在其中的作用。方法 :人脐带动脉平滑肌细胞体外原代培养 ,传至 4~ 5代用于实验。ox LDL干预 ,RT PCR测定VSMCMMP 9RNA和Lox 1mRNA表达。并观察予以Lox 1的抑制剂多聚肌苷酸作用后 ,VSMCMMP 9mRNA的表达的变化。结果 :①ox LDL作用后 ,VSMCMMP 9mRNA表达升高 ,P <0 .0 1,作用 1h出现峰值。ox LDL浓度升高 ,MMP 9mRNA表达增强。②ox LDL作用后 ,VSMCLox 1mRNA表达升高 ,且随着ox LDL浓度的升高有升高趋势 ,均P <0 .0 1。③多聚肌苷酸抑制Lox 1后 ,MMP 9mRNA表达明显下降 ,P <0 .0 1。结论 :ox LDL明显促进VSMCMMP 9表达 ,Lox 1在此过程中起重要介导作用
引用
收藏
页码:200 / 202
页数:3
相关论文
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