髓样分化因子88抑制剂和氧化低密度脂蛋白对单核巨噬细胞基质金属蛋白酶-8表达的影响

被引：1

作者：

杨红照

机构：

[1] 河南省西峡县人民医院心内科

来源：

中国实用医刊 | 2014年 / 41卷 / 14期

关键词：

髓样分化因子88抑制剂; 氧化低密度脂蛋白; 基质金属蛋白酶-8;

D O I：

暂无

中图分类号：

R543.5 [动脉疾病];

学科分类号：

1002 ; 100201 ;

摘要：

目的观察氧化低密度脂蛋白(oxLDL)和髓样分化因子88(MYD88)抑制剂对小鼠单核巨噬细胞RAW264.7炎症基因表达的影响。方法采用不同剂量的oxLDL刺激RAW264.7细胞,并用不同剂量MYD88抑制剂处理oxLDL 75μg/ml组,应用ELISA和逆转录-聚合酶链反应检测基质金属蛋白酶-8(MMP-8)及其mRNA表达的变化。结果 RAW264.7细胞经oxLDL刺激后MMP-8表达明显升高,且呈时间和剂量依赖性。MYD88抑制剂可抑制oxLDL刺激引起的RAW264.7细胞MMP-8表达升高。结论 MYD88抑制剂可逆转oxLDL诱导的RAW264.7细胞MMP-8高表达。

引用

共 10 条

[1] A Role of Matrix Metalloproteinase-8 in Atherosclerosis [J].

Laxton, Ross C. ;

Hu, Yanhua ;

Duchene, Johan ;

Zhang, Feng ;

Zhang, Zhongyi ;

Leung, Kit-Yi ;

Xiao, Qingzhong ;

Scotland, Ramona S. ;

Hodgkinson, Conrad P. ;

Smith, Katherine ;

Willeit, Johann ;

Lopez-Otin, Carlos ;

Simpson, Iain A. ;

Kiechl, Stefan ;

Ahluwalia, Amrita ;

Xu, Qingbo ;

Ye, Shu .

CIRCULATION RESEARCH, 2009, 105 (09) :921-U231

[2]

Rapid Endothelial Turnover in Atherosclerosis-Prone Areas Coincides With Stem Cell Repair in Apolipoprotein E–Deficient Mice[J] . Georgios Foteinos,Yanhua Hu,Qingzhong Xiao,Bernhard Metzler,Qingbo Xu. &nbspCirculation . 2008 (14)

[3]

Vascular inflammation and the renin-angiotensin system. Brasier AR,Recinos A,Eledrisi MS. Arteriosclerosis . 2002

[4]

Atherosclerosis:a classic inflammatory disease. Anogeianaki A,Angelucci D,Cianchetti E,D X0027 Alessandro M,Maccauro G,Saggini A,Salini V,Caraffa A,Tet X00e9 S,Conti F,Tripodi D,Shaik-Dasthagirisaheb YB. Int J Immunopalhol Pharmacol . 2011

[5]

Intraplaque MMP-8 levels are increased in asymptomatic patients with carotid plaque progression on ultrasound[J] . Marta Miguel Turu,Jerzy Krupinski,Esther Catena,Ana Rosell,Joan Montaner,Francisco Rubio,Jose Alvarez-Sabin,Marc Cairols,Lina Badimon. &nbspAtherosclerosis . 2005 (1)

[6]

Enhanced stabilization of atherosclerotic plaques in apolipoprotein E-knockout mice by combinatorial Toll-like receptor-1 and -2 gene silencing. Qi Li Hang,Wang Ying,Gao Fei,Zhang Cheng,Ding Shi Fang,Ni Mei,Feng Jin Bo,Zhang Yun. Human Gene Therapy . 2009

[7]

Atherosclerosis: A Classic Inflammatory Disease[J] . A. Anogeianaki,D. Angelucci,E. Cianchetti,M. D’alessandro,G. Maccauro,A. Saggini,V. Salini,A. Caraffa,S. Teté,F. Conti,D. Tripodi,Y.B. Shaik-Dasthagirisaheb. &nbspInternational Journal of Immunopathology and Phar . 2011 (4)

[8]

Expression of Neutrophil Collagenase (Matrix Metalloproteinase-8) in Human Atheroma: A Novel Collagenolytic Pathway Suggested by Transcriptional Profiling[J] . &nbspCirculation: Journal of the American Heart Association . 2001 (16)

[9] Inflammatory markers in coronary heart disease [J].

Madjid, Mohammad ;

Willerson, James T. .

BRITISH MEDICAL BULLETIN, 2011, 100 (01) :23-38

[10]

Serum matrix metalloproteinase-8 concentrations are associated with cardiovascular outcome in men. Tuomainen A M,Nyyssonen K,Laukkanen J A, et al. Arteriosclerosis . 2007

← 1 →