缺氧缺血新生鼠损伤脑组织中Nogo-A,Ng-R mRNA的表达

被引：6

作者：

王华

姚裕家

机构：

[1] 四川大学华西第二医院儿科,四川大学华西第二医院儿科成都,成都

来源：

郑州大学学报(医学版) | 2005年 / 02期

关键词：

缺氧缺血脑损伤; Nogo-A; Ng-R; mRNA;

D O I：

10.13705/j.issn.1671-6825.2005.02.042

中图分类号：

R722.1 [新生儿疾病];

学科分类号：

100202 ;

摘要：

目的:观察Nogo -A、Ng- RmRNA在缺氧缺血新生鼠损伤脑组织中表达量的变化。方法:将7d龄SD大鼠随机分为假手术组和缺氧缺血脑损伤(HIBD)组(结扎右颈动脉后,置于低氧舱处理),分别于缺氧处理0h、6h、 12h、24h、72h及7d后断头处死6只动物。用RT- PCR方法定量分析缺氧缺血侧脑组织Nogo A及Ng RmRNA的表达水平。结果:假手术组各时点脑组织Nogo -A及Ng -RmRNA表达无变化。与假手术组比较,HIBD组大鼠Nogo-AmRNA在HIBD后6h开始升高,12h达峰值;Ng- RmRNA在HIBD后6h开始升高,12h达峰值,24h时仍维持在较高水平。结论:Nogo -A、Ng -R可能与HIBD后神经再生抑制有一定关系。

引用

页码：305 / 307

页数：3

共 8 条

[1]

Oligodendrocyte-myelin glycoprotein is a Nogo receptor ligand that inhibits neurite outgrowth. Wang KC,Koprivica V,Kim JA,et al. Nature . 2002

[2]

Anti-Nogo-A antibody infusion 24 hours after experimental stroke improved behavioural outcome and corticospinal plasticity in normotensive and spontaneously hypertensive rats. Wiessner C,Bareyre FM,Allegrini PR,et al. Journal of Cerebral Blood Flow and Metabolism . 2003

[3]

Nogo-A is a myelin-associated neurite outgrowth inhibitor and an antigen for monoclonoal antibody IN-1. Chen MS,Huber AB,van der Haar ME,et al. Nature . 2000

[4]

Inhibitor of neurite outgrowth in humans. Prinjha R,Moore SE,Vinson M,et al. Nature . 2000

[5]

Identification of the Nogo inhibitor of axon regeneration as a reticulon protein. Grandpre T,Nakamura F,Vartanian T,et al. Nature . 2000

[6]

Identification of a receptor mediating Nogo-66 inhibition of axonal regeneration. Fournier AE,GrandPre T,Strittmatter SM. Nature . 2001

[7]

Long-lasting sprouting and gene expression changes induced by the monoclonal antibody IN-1 in the adult spinal cord. Bareyre FM,Handenschild B,Schwab ME. The Journal of Neuroscience . 2002

[8]

Myelin-associated glycoprotein as a functional ligand for the Nogo-66 receptor. Liu BP,Fournier A,GrandPre T,et al. Science . 2002

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