大鼠永久性大脑中动脉阻塞模型的建立

被引：10

作者：

许金秀

杨牧

周红

机构：

[1] 电子科技大学生命科学与技术学院,神经信息教育部重点实验室

来源：

四川动物 | 2010年 / 29卷 / 03期

关键词：

大鼠永久性大脑中动脉阻塞模型; TTC; 梗死体积;

D O I：

暂无

中图分类号：

R743.3 [急性脑血管疾病（中风）];

学科分类号：

1002 ;

摘要：

目的建立稳定可靠的线栓法大鼠大脑中动脉阻塞(MCAO)永久性脑缺血模型。方法对Koizumi和ZeaLonga模型构建方法加以改进。40只雄性SD大鼠随机均分为实验组和假手术组,分别进行模型制作和模型缺血的评价,采用2,3,5-氯化三苯四氮唑(2,3,5-Triphenyltetrazolium chloride,TTC)染色来计算大鼠脑梗死体积。结果大鼠MCAO模型制作成功率为95%;神经功能缺失评分1～3级百分率为95%。结论实验所采用的改良术式构建大鼠MCAO模型具有稳定性好、可重复率高、操作简便和手术时间短等优点,为基于大鼠永久性脑缺血模型开展的下游相关实验提供了良好的研究平台。

引用

页码：461 / 465+506 +506

页数：6

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