缺血缺氧对体外培养星形胶质细胞细胞周期和增殖的影响

被引：3

作者：

喻志源

王伟

阮旭中

机构：

[1] 华中科技大学同济医学院附属同济医院神经内科

[2] 华中科技大学同济医学院附属同济医院神经内科湖北武汉

[3] 湖北武汉

来源：

中风与神经疾病杂志 | 2003年 / 04期

关键词：

脑缺血; 星形胶质细胞; 细胞周期;

D O I：

暂无

中图分类号：

R741 [神经病学];

学科分类号：

1002 ;

摘要：

目的　观察缺血缺氧损伤对星形胶质细胞细胞周期和增殖的影响。方法　用流式细胞仪检测缺血缺氧后不同时间点星形胶质细胞细胞周期变化 ,并用荧光免疫细胞化学技术测定胶质细胞纤维酸性蛋白 (GFAP)和增殖细胞核抗原 (PCNA )的表达水平。结果　体外缺血缺氧损伤后星形胶质细胞 S期较正常组明显增高 ,6 h达高峰 ,而随后则呈下降趋势。PCNA阳性反应损伤后表达均增加 ,6 h表达最高 ;在缺血缺氧早期 ,GFAP阳性染色增强 ,6 h最高 ;缺血缺氧 12 h后 GFAP阳性染色变弱。结论　缺血缺氧损伤后星形胶质细胞活化进入增殖期 ;PCNA参与了损伤后星形胶质细胞的修复和增殖 ;细胞周期事件与星形胶质细胞的活化密切相关

引用

页码：27 / 29

页数：3

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