大鼠脑微血管分离及内皮细胞培养

被引：2

作者：

段重高 ^{[1
]}

修瑞娟 ^{[1
]}

机构：

[1] 中国医学科学院微循环研究所

来源：

中华医学杂志 | 1987年 / 11期

关键词：

脑血管; 细胞; 培养的; 动物; 实验;

D O I：

暂无

中图分类号：

R74 [神经病学与精神病学];

学科分类号：

1002 ;

摘要：

用匀浆、过滤及酶消化技术分离出大鼠脑微血管,建立脑微血管内皮细胞体外培养方法。通过相差显微镜观察,培养的活细胞为多边形扁平细胞,呈现接触性生长抑制特点和典型的"卵石"征象。间接免疫荧光染色法证明这些细胞95%以上是血管内皮细胞。

引用

共 11 条

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[8] Diabetes [P].

英国专利 :GB201117172D0 ,2011-11-16

[9]

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[10]

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