血小板激活因子及其拮抗剂银杏内酯B对大鼠心肌缺血再灌注损伤的影响(英文)

被引：5

作者：

焦向英

罗宁

支建明

赵荣瑞

机构：

[1] 山西医科大学生理学教研室,山西医科大学生理学教研室,山西医科大学生理学教研室,山西医科大学生理学教研室山西太原,山西太原,山西太原,山西太原

来源：

中国药理学与毒理学杂志 | 2002年 / 02期

关键词：

血小板激活因子; 银杏内酯B; 再灌注损伤; 缺血预适应; 心肌; 中性粒细胞; 凋亡;

D O I：

暂无

中图分类号：

R965 [实验药理学];

学科分类号：

100602 ; 100706 ;

摘要：

目的　探讨外源性血小板激活因子 (PAF)激活的多核形中性粒细胞 (PMN)是否具有低浓度氧自由基模拟缺血预适应 (IP)对缺血再灌注 (IR)所致心肌损伤的保护作用。方法　结扎冠脉左前降支 30min ,再灌注 3h制备大鼠心脏IR模型。以缺血前给予 2次 5min缺血 ,10min再灌注作为IP。实验分为6组 ,IR组、IR +PAF组和IR +银杏内酯B(GB、PAF拮抗剂 )组 ,分别于缺血前 2 5和 10miniv生理盐水、PAF(3μg·kg- 1)和GB (5mg·kg- 1) ;IP +IR组、IP +IR +PAF组和IP +IR +GB组分别于IP 2次 10min再灌注开始时iv相应药物。观察分析心功能、梗死面积、PMN计数、心肌髓过氧化物酶活性、TUNEL阳性细胞计数等指标。结果　给予PAF明显加重IR引起的心脏损伤、PMN浸润及凋亡的程度 ;GB对IR引起的心功能下降没有明显影响 ,但明显减少梗死面积、PMN浸润及凋亡细胞 ;PAF明显削弱IP的保护作用 ;GB对IP作用未见明显影响。结论　IR后PMN浸润可能是引起心肌细胞凋亡的一个重要原因 ;PAF激活PMN可能不具有模拟IP对IR所致心肌损伤的保护作用 ,反而取消IP的保护作用。

引用

页码：81 / 87

页数：7

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