BDNF,NGF在大鼠局灶性脑缺血的表达变化及葛根素对其影响的实验研究

被引：20

作者：

徐正东

李贯绯

机构：

[1] 吉林省人民医院神经内科

[2] 吉林省人民医院神经内科吉林长春

[3] 吉林长春

来源：

中国实验诊断学 | 2005年 / 04期

关键词：

脑缺血; 神经生长因子; 脑源性神经生长因子; 葛根素;

D O I：

暂无

中图分类号：

R741 [神经病学]; R285 [中药药理学];

学科分类号：

1002 ; 1008 ;

摘要：

目的观察脑缺血后脑源性神经营养因子(BDNF)及神经生长因子(NGF)含量的变化,并研究葛根素对大鼠局灶性脑缺血后脑内BDNF及NGF含量变化的影响及探讨其对缺血性脑损伤的神经元保护作用。方法拟用大鼠大脑中动脉线栓法(MCAO)建立缺血模型,采用免疫组织化学方法观察BDNF及NGF的表达。结果BDNF在MCAO后6h表达增强,1d达高峰,缺血组到3d达到对照组水平,治疗组在5d时仍有表达,到7d达到对照组水平。NGF在MCAO后1d达高峰并持续到7d,7d之后仍有少量表达,治疗组在14d仍有少量表达。结论脑缺血后神经元内BDNF和NGF的含量增多,可能有利于受损神经元的修复。葛根素可能通过促进BDNF、NGF的表达而保护神经元。

引用

页码：578 / 580

页数：3

共 7 条

[1]

Correlation between cerebral blood and histologyic changes in a new rat model of cerebral artery occlusion. Nagasawa H,Kogure K. Stroke . 1989

[2]

Reversible middle cerebral artery occlusion without craniectomy in rats. Zea longa EL,et al. Stroke . 1989

[3]

BDNF and NGF afford in vitro neuroprotection against ethanol combined with acute ischemia and chronic hypoglycemia. Mitchell JJ,Paiva M,Walker DW,et al. Developmental Neuroscience . 1999

[4]

Rapid alterations of BDNFprotein levels in the rat brain after focal ischemia: evidence for increased systhesis and anterograde axonal transport. Kokaia Z,Andsberg G,Yan Q,et al. Experimental Neurology . 1998

[5]

Neuroprotective effects of estradiol in mesencephalic dopaminergic neuron. Sawada H,Shimobama S. Neuroscience and Biobehavioral Reviews . 2000

[6]

Estradio protects mesencephalic dopaminergic neurons from oxidative stress-induced-neuronal death. Sawada H,Ibi M,Kihara T,et al. Journal of Neuroscience Research . 1998

[7]

Brain derived neurotrophic factor transgenic mice exhibit passive avoidance deficits, increased seizure severity and in vitro hyperexcitability in the hippocampus and entorhinal corex. Croll SD,Suri C,Compton DL,et al. Neuroscience . 1999

← 1 →