多巴胺诱导PC12细胞凋亡的机制

被引：3

作者：

陈晓春

朱理安

黄春

机构：

[1] 福建医科大学附属协和医院、福建省老年医学研究所!福州

来源：

福建医科大学学报 | 2000年 / 02期

关键词：

多巴胺; 大鼠嗜铬细胞瘤细胞株; 凋亡; 帕金森病;

D O I：

暂无

中图分类号：

R96 [药理学];

学科分类号：

100602 ; 100706 ;

摘要：

目的　探讨多巴胺 (DA)诱导大鼠嗜铬细胞瘤细胞株 (PC12 )细胞凋亡的机制。　方法　采用 PC12细胞为模型 ,以 0 .45 mmol/ L的 DA来诱导细胞凋亡的发生 ,用流式细胞仪检测 PC12细胞的亚二倍体峰 ,按 Griess法测定 NO代谢产物 NO2 -的浓度 ,用 Ac- DEVD- AMC为酶作用底物 ,在荧光分光光度计下检测 CPP32活力。　结果　 DA处理组的凋亡率为 5 4.49%± 1.6 0 % ,与阴性对照组 (1.6 1%± 0 .18% )比较差异有显著性 (P<0 .0 1) ;NO2 -浓度的升高和 CPP32活化程度与阴性对照组比较差异亦有显著性 (P<0 .0 1)。　结论　 CPP32的激活是 DA诱导 PC12细胞凋亡的重要通路 ,而合成增多的 NO可能是 DA激活 CPP32的信号传递分子。

引用

页码：93 / 95

页数：3

共 10 条

[1]

Modulation of control mechanisms of dopamine-induced apoptosis: a future approach to the treatment of Parkinson’ s disease. Ziv I,Offen D,Barzilai A,et al. Journal of Neural Transmission . 1997

[2]

Group I metabotropic receptors downregulate nitric oxide induced caspase-3 activity in rat hippocampal neurons. Maiese K,Vincent AM. Neuroscience Letters . 1999

[3]

Apoptotic-like changes in Lewy-body-associated disorders and normal aging in substantia nigral neurons. Tomkins MM,Basgill EJ,Zamrini E,et al. American Journal of Pathology . 1997

[4]

Apoptosis and autopathy in nigral neurons of patients with Parkinson’ s disease. Anglade P,Vyas S,Javoy-Agid F,et al. Histology and Histopathology . 1997

[5]

Prevention of dopamineinduced cell death by thionyl antioxidants: possible implications for treatment of Parkinson’ s disease. Offen D,Ziv I,Stermin H,et al. Experimental Neurology . 1996

[6]

Activation of c-jun N-terminal kinases stress-activated protein kinase and the decreased ratio of Bcl -2 to Bax are associated with the auto-oxidized dopamine-induced apoptosis in PC12 cells. Kang C,Jang J,Kim K,et al. Neuroscience Letters . 1998

[7]

Dopamine induces apoptosis through an oxidation-involved SAPK JNK activation pathway. Luo Y,Umegaki H,Wang X,et al. Journal of Biological Chemistry . 1998

[8]

Histochemical detection of apoptosis in Parkinson’ s disease. Mochizuki H,Goto K,Mori H,et al. Journal of the Neurological Sciences . 1996

[9]

Nitric oxide is involved in apoptosis induced by thapsigargin in rat mesangial cells. Rodriguez-Lopez AM,Martinez-Salgado C,Eleno N,et al. Cellular Physiology and Biochemistry . 1999

[10]

Tumor necrosis factoralpha and phorbol 12-myristate 13-acetate differentially modulate cytotoxic effect of nitric oxide generated by serum deprivation in neuronal PC12 cells. Chung KC,Park JH,Kim CH,et al. Journal of Neurochemistry . 1999

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