β-淀粉样蛋白影响维甲类X受体α到细胞质的穿梭

被引：2

作者：

游晓青

陈耀民

黄秀梅

刘迎春

林建银

机构：

[1] 福建医科大学基础医学院细胞生物学与遗传学系

[2] 美国加州Burnham医学研究院

[3] 福建医科大学基础医学院,病原生物学系

来源：

中华神经科杂志 | 2011年 / 10期

关键词：

阿尔茨海默病; 淀粉样β蛋白; 维甲类X受体α; 细胞质;

D O I：

暂无

中图分类号：

R749.16 [];

学科分类号：

100203 ;

摘要：

目的探讨β-淀粉样蛋白(Ap)对维甲类X受体α(RXRα)生成及到细胞质迁移的影响。方法将野生型小鼠脑神经瘤细胞(N2awt)分为对照组和实验组,采用AB短肽及淀粉样肽前体蛋白(APP)695过表达处理实验组,应用核质分离结合Western blot方法,检测RXRα在细胞核和细胞质中的含量;分别对上述2组N2awt细胞及阿尔茨海默病(AD)患者及健康对照者的脑组织切片进行免疫荧光定位染色,观察RXRα在细胞核与细胞质中分布的改变。结果在N2awt细胞中,APP或AB增多不影响RXRα的表达但导致RXRα在细胞质中含量与分布增多,分别从对照组的3.2%增加至APP处理组的17.6%及从对照组的3.8%增加至AB处理组的14.3%;与健康人比较,AD患者脑皮质细胞中RXRα在细胞质分布增加。结论 AB可能影响RXRα到细胞质的穿梭。

引用

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