NOTE ON THE PRETREATMENT OF EXCISED FLOWER BUDS IN FLOAT CULTURE OF HYOSCYAMUS ANTHERS

被引:11
作者
SUNDERLAND, N
WILDON, DC
机构
[1] John Innes Institute, Norwich, Colney Lane
来源
PLANT SCIENCE LETTERS | 1979年 / 15卷 / 02期
关键词
D O I
10.1016/0304-4211(79)90070-1
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
As a means of enhancing the embryogenic response of Hyoscyamus pollen in anther culture, excised bud pretreatment is shown to be more effective at 15°C than at 7.5°C. Pretreatment at 5°C is ineffectual. The pretreatment optimum at 15°C is 5 days compared to 15 days at 7.5°C. A greater response is invoked by bud pretreatment than by excised anther pretreatment. Without pretreatment, the anthers are most responsive when cultured just before the first mitotic division of the pollen, but with pretreatment, they become most responsive at or just after the first mitotic division. The pretreatment-induced response in Hyoscyamus is compared with that in Nicotiana tabacum. © 1979.
引用
收藏
页码:169 / 175
页数:7
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