THE MULTIDOMAIN STRUCTURE OF ORC1P REVEALS SIMILARITY TO REGULATORS OF DNA-REPLICATION AND TRANSCRIPTIONAL SILENCING

被引：222

作者：

BELL, SP ^{[1
]}

MITCHELL, J ^{[1
]}

LEBER, J ^{[1
]}

KOBAYASHI, R ^{[1
]}

STILLMAN, B ^{[1
]}

机构：

[1] MIT,DEPT BIOL,CAMBRIDGE,MA 02139

来源：

CELL | 1995年 / 83卷 / 04期

关键词：

D O I：

10.1016/0092-8674(95)90096-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The origin recognition complex (ORC) is a six protein assembly that binds S. cerevisiae origins of replication and directs DNA replication throughout the genome and transcriptional silencing at the yeast mating-type loci. Here we report the cloning of the genes encoding the 120 kDa (ORC1), 62 kDa (ORC3), and 56 kDa (ORC4) subunits of ORC and the reconstitution of the complete complex after expression of all six subunits in insect cells. Orc1p is related to Cdc6p and Cdc18p, which regulate DNA replication and mitosis, and to Sir3p, a regulator of transcriptional silencing. The N-terminal region of Orc1p is highly related to Sir3p, and studies of Orc1p/Sir3p chimeric proteins indicate that this domain is dedicated to the transcriptional silencing function of ORC.

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页码：563 / 568

页数：6

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