STEROIDS IN GERMFREE AND CONVENTIONAL RATS - FREE STEROIDS IN FAECES FROM CONVENTIONAL RATS

被引：30

作者：

ERIKSSON, H

GUSTAFSSON, JA

SJOVALL, J

机构：

[1] Kemiska Institutionen, Karolinska Institutet, Stockholm, 10401

[2] Institutionen for Medicinsk Symbios Forskning, Kemiska Institutionen, Karolinska Institutet, Stockholm, S-10401

来源：

EUROPEAN JOURNAL OF BIOCHEMISTRY | 1969年 / 9卷 / 02期

关键词：

D O I：

10.1111/j.1432-1033.1969.tb00607.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Free steroids in faeces from germfree and conventional rats were studied by gas chromato‐graphy‐mass spectrometry after purification on carboxymethyl Sephadex LH‐20 and separation on methylated Sephadex G‐25. Only minor amounts of free steroids were found in germfree rats; these could not be identified. In contrast to this, conventional rats excreted considerable amounts of free steroids: 3α‐hydroxy‐5α,17α‐pregnan‐20‐one, 3α‐hydroxy‐5α‐pregnan‐20‐one, 3β‐hydroxy‐5α,17α‐pregnan‐20‐one (major component), 3β‐hydroxy‐5α‐pregnan‐20‐one, and 5α‐pregnane‐3β,20β‐diol. Furthermore, 5α‐pregnane‐3α,20β‐diol was tentatively identified. These findings are discussed in relation to what is known about steroid sulphates in faeces from germfree and conventional rats. Copyright © 1969, Wiley Blackwell. All rights reserved

引用

页码：286 / +

页数：1

共 12 条

[1]

ADLERCREUTZ H, 1966, EUR J STEROIDS, V1, P117

[2] QUANTIFICATION OF CHOLESTERYL SULFATE AND NEUTRAL STEROL DERIVATIVES IN HUMAN FECES AFTER PURIFICATION ON LIPOPHILIC SEPHADEX GELS - BILE ACIDS AND STEROIDS 188 [J].