Immunological evidence that HMG-CoA reductase kinase-A is the cauliflower homologue of the RKIN1 subfamily of plant protein kinases

被引：46

作者：

Ball, KL

Barker, J

Halford, NG

Hardie, DG

机构：

[1] UNIV DUNDEE,DEPT BIOCHEM,DUNDEE DD1 4HN,SCOTLAND

[2] UNIV BRISTOL,DEPT AGR SCI,LONG ASHTON RES STN,IACR,BRISTOL BS18 9AF,AVON,ENGLAND

来源：

FEBS LETTERS | 1995年 / 377卷 / 02期

基金：

英国生物技术与生命科学研究理事会;

关键词：

HMG-CoA reductase kinase; AMP-activated protein kinase; SNF1; RKIN1; BKIN12; AKIN10;

D O I：

10.1016/0014-5793(95)01343-1

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Three different antibodies against the RKIN1 and BKIN12 gene products from rye and barley recognized the 58 kDa subunit of HRIIG-CoA reductase kinase-A (HRK-A) from Brassica oleracea on Western blots, HRK-A was also detected by an antipeptide antibody in enzyme-linked immunoassays, and this was competed by the peptide antigen. HRK-A was not recognized by antibodies against plant, mammalian and Saccharomyces ces cerevisiae relatives of RKIN1, i.e. wheat PKABA1, rat AMP-activated protein kinase and S. cerevisiae Snf1p. RKIN1/HMG-CoA reductase kinase-A are now among the first protein kinases in plants to be web characterized at both the molecular and biochemical levels.

引用

收藏

页码：189 / 192

页数：4

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