EFFECT OF BREATHING OXYGEN ON METABOLISM OF RAT BRAIN UNDER NORMAL AND ISCHAEMIC CONDITIONS

Abstract— Breathing oxygen (1 atm.) for 2 hr increased the glycogen content of the rat brain from 3·38 to 4·35 μmoles glucosyl residues/g wet wt. At the same time the glucose and lactate concentrations were significantly decreased. Both under normal conditions and when breathing oxygen, the sum (glycogen + glucose) × 2 + lactate, with which the balance of carbohydrate breakdown and lactate formation was assessed, was 13·5 μmoles/g wet wt. Oxygen breathing effected a significant decrease in this sum after an ischaemic period of 1–15 min. In the control group breathing normal air, the sum, after all periods of ischaemia, ranged from 98 to 106 per cent of the starting value. An increased partial pressure of oxygen did not change the breakdown rate of the high‐energy phosphate compounds. This result was not consistent with an oxidation of the carbohydrates which were missing in the balance. It is probable that other metabolites, which were not tested for, accumulated. 0 We failed to find any indication of storage of oxygen which the ischaemic brain could use for oxidative energy production. Copyright © 1969, Wiley Blackwell. All rights reserved