FLUORESCENCE BLEACH RATE IMAGING

被引:23
作者
BRAKENHOFF, GJ
VISSCHER, K
GIJSBERS, EJ
机构
[1] Section of Molecular Cytology, Department of Molecular Cell Biology, University of Amsterdam, Amsterdam, NL 1018 TV
来源
JOURNAL OF MICROSCOPY-OXFORD | 1994年 / 175卷
关键词
FLUORESCENCE MICROSCOPY; PHOTOBLEACHING; BLEACH RATE; BLEACH MAP; HISTOMAP; CONFOCAL; BILATERAL; RATIO-IMAGING; CCD; PHOTODEGRADATION;
D O I
10.1111/j.1365-2818.1994.tb03478.x
中图分类号
TH742 [显微镜];
学科分类号
摘要
Bleach rate imaging on a (cooled) CCD can be easily achieved using a confocal microscope with bilateral scanning and detection coupled to a workstation; it is as easy as acquiring regular fluorescence images. Several analysis and display methods for bleach rate imaging are presented such as the bleach map (and its inverse) and a matrix-based decomposition method for multi-labelled specimens based on the bleach rate differences between the dyes used. With these tools, bleach-rate-based imaging can become a viable alternative to multiple labelling techniques for component identification in fluorescent specimens.
引用
收藏
页码:154 / 161
页数:8
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