MAXI-CIRCLE OF TRYPANOSOMA-BRUCEI KINETOPLAST DNA

被引：60

作者：

BORST, P

FASEFOWLER, F

机构：

[1] Section of Medical Enzymology and Molecular Biology, Laboratory of Biochemistry, University of Amsterdam, 1005 GA Amsterdam

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1979年 / 565卷 / 01期

关键词：

(Trypanosoma brucei); DNA mapping; Kinetoplast DNA; Mitochondrial DNA;

D O I：

10.1016/0005-2787(79)90078-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have constructed a fragment map of the maxi-circle component from kinetoplast DNA networks of Trypanosoma brucei brucei (East African Trypanosomiasis Research Organization strain No. 427), using restriction endonucleases PstI, HapII, EcoRI, BglI, HindIII, HhaI, SstI, XbaI, HaeIII and Sau-96I. Although the 20 kilo-base pair map contains 30 fragments, there is a 6.5 kilo-base pair 'silent' segment which lacks recognition sites for any of these enzymes. In CsCl intact kinetoplast DNA has a buoyant density of 1.690 g/cm3, whereas a kinetoplast DNA fraction enriched in maxi-circle sequences contains an additional component at 1.682 g/cm3. The possibility that the 'silent' segment is very rich in A + T is discussed. © 1979.

引用

页码：1 / 12

页数：12

共 19 条

[1] MITOCHONDRIAL DNA .I. PREPARATION AND PROPERTIES OF MITOCHONDRIAL DNA FROM CHICK LIVER [J].

BORST, P ;

RUTTENBERG, GJ ;

KROON, AM .

BIOCHIMICA ET BIOPHYSICA ACTA, 1967, 149 (01) :140-+

[2]

Borst P., 1976, P169

[3]

BORST P, 1979, SPECIFIC EUKARYOTIC, P294

[4]

BORST P, 1976, GENETIC FUNCTION MIT, P59

[5]

BORST P, 1976, HDB BIOCHEMISTRY MOL, V2, P363

[6] CLEAVAGE OF POLYOMA-VIRUS DNA BY RESTRICTION ENZYMES KPNI AND PSTI [J].

CRAWFORD, LV ;

ROBBINS, AK .

JOURNAL OF GENERAL VIROLOGY, 1976, 31 (JUN) :315-321

[7] NEW METHOD FOR ISOLATION OF A RESTRICTION ENZYME FROM HEMOPHILUS PARAINFLUENZAE [J].

DEFILIPPES, FM .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1974, 58 (03) :586-596

[8] ISOLATION AND CHARACTERIZATION OF KINETOPLAST DNA FROM BLOOD-STREAM FORM OF TRYPANOSOMA-BRUCEI [J].

FAIRLAMB, AH ;

WEISLOGEL, PO ;

HOEIJMAKERS, JHJ ;

BORST, P .

JOURNAL OF CELL BIOLOGY, 1978, 76 (02) :293-309

[9] ORIGIN AND DIRECTION OF REPLICATION IN MITOCHONDRIAL-DNA MOLECULES FROM DROSOPHILA-MELANOGASTER [J].

GODDARD, JM ;

WOLSTENHOLME, DR .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1978, 75 (08) :3886-3890

[10] ANALYSIS OF PHI-29 AND PHI-15 GENOMES BY BACTERIAL RESTRICTION ENDONUCLEASES, ECOR1 AND HPAL [J].

ITO, J ;

KAWAMURA, F ;

YANOFSKY, S .

VIROLOGY, 1976, 70 (01) :37-51

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