BIOCHEMICAL STUDIES ON LIPOPOLYSACCHARIDES OF SALMONELLA R MUTANTS .3. LINKAGE OF HEPTOSE UNITS

被引：47

作者：

DROGE, W

LUDERITZ, O

WESTPHAL, O

机构：

[1] Max-Max-Planck-Institut für Immunbiologie, Freiburg Im Breisgau

来源：

EUROPEAN JOURNAL OF BIOCHEMISTRY | 1968年 / 4卷 / 01期

关键词：

D O I：

10.1111/j.1432-1033.1968.tb00182.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

From Salmonella R lipopolysaccharides of the chemotypes Rc, Rd1, and Rd2 oligosaccharides were prepared and their structure studied by the following methods: quantitative sugar analyses; periodate oxidation followed by borohydride reduction in order to transform the l‐glycero‐d‐mannose residues into d‐mannose residues; methylation of original and transformed oligosaccharides, methanolysis and gas chromatographic identification of the methylated heptoses or mannoses; the action of glycosidases; serological tests. The results of the study are in agreement with the formulae given below for the different oligosaccharides. The anomeric form of the glucose unit linked to heptose, as well as that part of the oligosaccharides which contains the 2‐keto‐3‐deoxy‐octonate residues [KDO] remain uncertain. Chemotypes Rd2: Heptose pα 1→[KDO]x; Rd1: Heptose pα 1→3 Heptose pα 1→[KDO]x; Re: Glucose 1→3 Heptose pα 1→3 Heptose pα 1→[KDO]x. Copyright © 1968, Wiley Blackwell. All rights reserved

引用

页码：126 / &

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LUDERITZ, O ;

GALANOS, C ;

RISSE, HJ ;

RUSCHMANN, E ;

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SCHMIDT, G ;

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WHEAT, R ;

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ANNALS OF THE NEW YORK ACADEMY OF SCIENCES-SERIES, 1966, 133 (A2) :349-+

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