ATP-INDUCED STIMULATION OF CALCIUM-BINDING TO CARDIAC SARCOLEMMA

被引：25

作者：

MASOLIVA, J

WILLIAMS, AJ

NAYLER, WG

机构：

[1] Cardiothoracic Institute, University of London, London, W1N 2DX England, 2, Beaumont Street

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1979年 / 87卷 / 02期

关键词：

D O I：

10.1016/0006-291X(79)91815-1

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Sarcolemma vesicles isolated from rabbit ventricular muscle showed a high degree of purity when assessed in terms of calcium binding and marker enzyme activity. Calcium binding to the sarcolemma increased as the free calcium concentration of the medium increased. ATP-independent (passive) and ATP-dependent (active) calcium binding activities were observed. The active binding was associated with an increase in the calcium stimulated ATPase activity of the preparation. A Lineweaver-Burke plot of the ATP-dependent calcium binding data was linear over the whole range of calcium concentrations (20-200 μM) investigated. © 1979.

引用

页码：441 / 447

页数：7

共 18 条

[1]

BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3

[2]

COOPERSTEIN SJ, 1951, J BIOL CHEM, V189, P665

[3] CALCIUM-BINDING AND ATPASE ACTIVITIES OF HEART SARCOLEMMA [J].