SOMATOSTATIN ACTIVATES PARTICULATE GUANYLATE-CYCLASE IN CULTURED RAT MESANGIAL CELLS

被引:11
作者
GARCIAESCRIBANO, C
DIEZMARQUES, ML
MEDINAALONSO, J
RODRIGUEZPUYOL, M
RODRIGUEZPUYOL, D
机构
[1] HOSP PRINCIPE ASTURIAS,NEPHROL SECT,E-28805 MADRID,SPAIN
[2] ALCALA DE HENARES UNIV,DEPT PHYSIOL,MADRID,SPAIN
[3] ALCALA DE HENARES UNIV,DEPT PHARMACOL,MADRID,SPAIN
[4] ALCALA DE HENARES UNIV,DEPT MED,MADRID,SPAIN
关键词
D O I
10.1038/ki.1994.459
中图分类号
R5 [内科学]; R69 [泌尿科学(泌尿生殖系疾病)];
学科分类号
1002 ; 100201 ;
摘要
Although the ability of somatostatin (ST) to relax cultured rat mesangial cells has recently been described, the intimate cellular mechanisms responsible for this effect have not been adequately clarified. The present experiments were designed to test the hypothesis that cyclic GMP (cGMP) could be involved in the genesis of this relaxation. ST increased cGMP synthesis by cultured rat mesangial cells, in basal conditions and in the presence of isobutylmethylxanthine or zaprinast. This effect was dose-dependent, with a threshold value of about 1 nM and a maximal response at ST concentrations between 0.1 and 1 mu M. This increased cGMP synthesis was dependent on the stimulation by ST of a particulate guanylate cyclase, as the synthesis of cGMP by a particulate membrane fraction obtained from the cells increased in the presence of ST. When the cGMP-specific phosphodiesterase of mesangial cells was blacked with zaprinast, the ST-dependent relaxation, assessed both by morphological and biochemical criteria, significantly increased with respect to the experiments performed without zaprinast. These results support a role for cGMP in the ST-dependent relaxation of cultured rat mesangial cells. The increased cGMP synthesis appears to be the consequence of the activation of some form of particulate guanylate cyclase.
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页码:1611 / 1615
页数:5
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