IMMUNOCYTOCHEMISTRY WITH OSMIUM-FIXED TISSUE .1. LIGHT MICROSCOPIC LOCALIZATION OF GROWTH-HORMONE AND PROLACTIN WITH THE UNLABELED ANTIBODY-ENZYME METHOD

被引：111

作者：

BASKIN, DG ^{[1
]}

ERLANDSEN, SL ^{[1
]}

PARSONS, JA ^{[1
]}

机构：

[1] UNIV MINNESOTA, SCH MED, DEPT ANAT, MINNEAPOLIS, MN 55455 USA

来源：

JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY | 1979年 / 27卷 / 04期

关键词：

D O I：

10.1177/27.4.109497

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Growth hormone [GH] and prolactin [PRL] were localized on thin plastic sections of rat anterior pituitary gland and mammosomatotropic tumor MtTW15 that were fixed with osmium tetroxide (alone, mixed with aldehydes, or after aldehydes). Intense immunocytochemical staining for both antigens was obtained after plastic was removed from sections with an alcoholic solution of NaOH. Antigenic determinants of rat PRL and GH were not completely destroyed or inactivated by fixation with osmium and embedment in epoxy resin and removal of the polymerized epoxy resin was necessary to obtain light microscopic postembedding immunocytochemical staining of these antigens. Tissues which have been conventionally processed for morphological evaluation by EM may be suitable for postembedding immunocytochemical staining of some antigens for light microscopy.

引用

页码：867 / 872

页数：6

共 38 条

[1] CROSS-LINKING OF PROTEINS WITH GLUTARALDEHYDE AND ITS USE FOR PREPARATION OF IMMUNOADSORBENTS [J].

AVRAMEAS, S ;

TERNYNCK, T .

IMMUNOCHEMISTRY, 1969, 6 (01) :53-+

[2] GENESIS OF CELL-TYPES IN ADENOHYPOPHYSIS OF HUMAN FETUS AS OBSERVED WITH IMMUNOCYTOCHEMISTRY [J].

BAKER, BL ;

JAFFE, RB .

AMERICAN JOURNAL OF ANATOMY, 1975, 143 (02) :137-161

[3]

BASKIN D G, 1978, Journal of Histochemistry and Cytochemistry, V26, P224

[4]

BEAUVILLAIN JC, 1977, CELL TISSUE RES, V184, P343, DOI 10.1007/BF00219895

[5] SELECTIVE STAINING OF NERVOUS TISSUE FOR LIGHT MICROSCOPY FOLLOWING PREPARATION FOR ELECTRON MICROSCOPY [J].

BERKOWITZ, LR ;

FIORELLO, O ;

KRUGER, L ;

MAXWELL, DS .

JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY, 1968, 16 (12) :808-+

[6] PREPARATION OF BIOLOGICAL TISSUES FOR ELECTRON-MICROSCOPY BY FREEZE-DRYING [J].

COULTER, HD ;

TERRACIO, L .

ANATOMICAL RECORD, 1977, 187 (04) :477-493

[7]

COULTER HD, 1978, ANAT REC, V190, P369

[8] PITUITARY ADENOMA PRODUCING THYROTROPIN AND PROLACTIN - IMMUNOCYTOCHEMICAL AND ELECTRON-MICROSCOPIC STUDY [J].

DUELLO, TM ;

HALMI, NS .

VIRCHOWS ARCHIV A-PATHOLOGICAL ANATOMY AND HISTOPATHOLOGY, 1977, 376 (03) :255-265

[9] EFFECT OF CYPROTERONE-ACETATE ON CELLS OF PARS-DISTALIS OF ADENOHYPOPHYSIS IN BEAGLE BITCH [J].

ELETREBY, MF ;

FATHELBAB, MR .

CELL AND TISSUE RESEARCH, 1977, 183 (02) :177-189

[10] MODIFICATION OF UNLABELED ANTIBODY ENZYME METHOD USING HETEROLOGOUS ANTISERA FOR LIGHT MICROSCOPIC AND ULTRASTRUCTURAL LOCALIZATION OF INSULIN, GLUCAGON AND GROWTH-HORMONE [J].

ERLANDSEN, SL ;

PARSONS, JA ;

BURKE, JP ;

REDICK, JA ;

VANORDEN, DE ;

VANORDEN, LS .

JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY, 1975, 23 (09) :666-677

← 1 2 3 4 →