PRESENCE OF A POTENT TRANSCRIPTION ACTIVATING SEQUENCE IN THE P53 PROTEIN

被引：873

作者：

FIELDS, S

JANG, SK

机构：

[1] Department of Microbiology, School of Medicine, State University of New York at Stony Brook, Stony Brook

来源：

SCIENCE | 1990年 / 249卷 / 4972期

关键词：

D O I：

10.1126/science.2144363

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The p53 gene is frequently mutated in a wide variety of human cancers. However, the role of the wild-type p53 gene in growth control is not known. Hybrid proteins that contain the DNA binding domain of yeast GAL4 and portions of p53 have been used to show that the p53 protein contains a transcription-activating sequence that functions in both yeast and mammalian cells. The NH2-terminal 73 residues of p53 activated transcription in mammalian cells as efficiently as the herpes virus protein VP16, which contains one of the strongest known activation domains. Combined with previous data that showed p53 is localized to the nucleus and can bind to DNA, these results support the idea that one function of p53 is to activate the transcription of genes that suppress cell proliferation.

引用

页码：1046 / 1049

页数：4

共 38 条

[1] DETECTION OF A COMMON FEATURE IN SEVERAL HUMAN-TUMOR CELL-LINES - A 53,000-DALTON PROTEIN - (SIMIAN-VIRUS 40 TUMOR ANTIGEN-ASSOCIATED HOST PROTEIN-PHOSPHOPROTEIN-MONOCLONAL ANTIBODY-IMMUNOPRECIPITATION) [J].