USE OF A CENTROMERE-SPECIFIC DNA PROBE (P82H) IN NONISOTOPIC INSITU HYBRIDIZATION FOR CLASSIFICATION OF MICRONUCLEI

被引：75

作者：

BECKER, P ^{[1
]}

SCHERTHAN, H ^{[1
]}

ZANKL, H ^{[1
]}

机构：

[1] UNIV KAISERSLAUTERN,DEPT BIOL,DIV HUMAN GENET,POB 3049,W-6750 KAISERSLAUTERN,GERMANY

来源：

GENES CHROMOSOMES & CANCER | 1990年 / 2卷 / 01期

关键词：

D O I：

10.1002/gcc.2870020111

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

The DNA probe p82H was used to visualize centromeric DNA in micronuclei (MN) of human cells. Slides prepared from cultures treated by the aneugen (causing aneuploidy) colcemid showed significantly more MN with centromeric signals than those treated by the clastogen (causing chromosome breakage) bleomycin. These results indicate that in situ hybridization with the alphoid p82H DNA probe is a suitable method with which to distinguish between MN containing whole chromosomes and acentric fragments, and hence allows one to discriminate between the clastogenic and aneugenic effects in MN formation. Copyright © 1990 Wiley‐Liss, Inc., A Wiley Company

引用

页码：59 / 62

页数：4

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