6DMAP INHIBITS CHROMATIN DECONDENSATION BUT NOT SPERM HISTONE KINASE IN SEA-URCHIN MALE PRONUCLEI

被引：32

作者：

POCCIA, D ^{[1
]}

PAVAN, W ^{[1
]}

GREEN, GR ^{[1
]}

机构：

[1] JOHNS HOPKINS UNIV,SCH MED,BIOCHEM CELLULAR & MOLEC BIOL TRAINING PROGRAM,BALTIMORE,MD 21205

来源：

EXPERIMENTAL CELL RESEARCH | 1990年 / 188卷 / 02期

关键词：

D O I：

10.1016/0014-4827(90)90164-6

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Treatment of sea urchin eggs for 10 min prior to fertilization with the kinase inhibitor 6DMAP (6-dimethylaminopurine) reversibly inhibits swelling and loss of conical morphology of the male pronucleus. Male pronuclei inhibited with 1 mM 6DMAP for 25 min undergo phosphorylation of Sp H1 and Sp H2B histones as fully as do control nuclei. Therefore, Sp histone kinase, whose target sequences resemble those of the M-phase histone kinase, is not inhibited by 6DMAP, and Sp histone phosphorylation, although it may be necessary, is not sufficient for chromatin decondensation. © 1990.

引用

页码：226 / 234

页数：9

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