REGULATION OF UCP GENE-EXPRESSION IN BROWN ADIPOCYTES DIFFERENTIATED IN PRIMARY CULTURE - EFFECTS OF A NEW BETA-ADRENOCEPTOR AGONIST

被引：57

作者：

CHAMPIGNY, O ^{[1
]}

HOLLOWAY, BR ^{[1
]}

RICQUIER, D ^{[1
]}

机构：

[1] ICI PLC,DEPT BIOSCI 2,MACCLESFIELD SK10 4TG,CHESHIRE,ENGLAND

来源：

MOLECULAR AND CELLULAR ENDOCRINOLOGY | 1992年 / 86卷 / 1-2期

关键词：

BROWN ADIPOCYTE; CELL CULTURE; UNCOUPLING PROTEIN; ADRENOCEPTOR;

D O I：

10.1016/0303-7207(92)90177-8

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Primary cultures of precursor cells from mouse and rat brown adipose tissue (BAT) were used to study the effect of a new beta-agonist (ICI D7114) on the uncoupling protein (UCP) gene expression. ICI 215001 (thc active metabolite of D7114) increased the expression of UCP and its mRNA in brown adipocytes differentiating in vitro in a dose-dependent manner. This stimulating effect was not inhibited by propranolol, a non-specific-beta-antagonist, but was partially reduced by bupranolol, a beta-3-antagonist. No expression of UCP mRNA was ever induced by ICI 215001 in white adipocytes differentiated in vitro. It was concluded that the drug could affect the brown adipose cells through a beta-3-pathway. It could clearly modulate the expression of UCP in brown adipocytes differentiated in vitro. but was not able by itself to turn on the gene.

引用

页码：73 / 82

页数：10

共 43 条

[1] COUPLING OF GROWTH ARREST AND EXPRESSION OF EARLY MARKERS DURING ADIPOSE CONVERSION OF PREADIPOCYTE CELL-LINES [J].

AMRI, EZ ;

DANI, C ;

DOGLIO, A ;

GRIMALDI, P ;

AILHAUD, G .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1986, 137 (02) :903-910

[2] THE BROWN ADIPOCYTE BETA-ADRENOCEPTOR [J].

ARCH, JRS .

PROCEEDINGS OF THE NUTRITION SOCIETY, 1989, 48 (02) :215-223

[3] ATYPICAL BETA-ADRENOCEPTOR ON BROWN ADIPOCYTES AS TARGET FOR ANTI-OBESITY DRUGS [J].

ARCH, JRS ;

AINSWORTH, AT ;

CAWTHORNE, MA ;

PIERCY, V ;

SENNITT, MV ;

THODY, VE ;

WILSON, C ;

WILSON, S .

NATURE, 1984, 309 (5964) :163-165

[4]

ARCH JRS, 1987, RECENT ADV OBESITY R, V5, P300

[5] DIBUTYRYL-CYCLIC-AMP DECREASES THE RATE OF LIPOPROTEIN-LIPASE SYNTHESIS IN CULTURED ADIPOCYTES [J].

BENSADOUN, A ;

MARITA, RA .

BIOCHIMICA ET BIOPHYSICA ACTA, 1986, 879 (03) :253-263

[6] SEQUENCE AND GENE ORGANIZATION OF MOUSE MITOCHONDRIAL-DNA [J].

BIBB, MJ ;

VANETTEN, RA ;

WRIGHT, CT ;

WALBERG, MW ;

CLAYTON, DA .

CELL, 1981, 26 (02) :167-180

[7] MOLECULAR APPROACH TO THERMOGENESIS IN BROWN ADIPOSE-TISSUE - CDNA CLONING OF THE MITOCHONDRIAL UNCOUPLING PROTEIN [J].

BOUILLAUD, F ;

RICQUIER, D ;

THIBAULT, J ;

WEISSENBACH, J .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1985, 82 (02) :445-448

[8]

BRAWERMAN G, 1983, METHOD CELL BIOL, V7, P1

[9] COLD-INDUCED BETA-ADRENERGIC RECRUITMENT OF LIPOPROTEIN-LIPASE IN BROWN FAT IS DUE TO INCREASED TRANSCRIPTION [J].

CARNEHEIM, C ;

NEDERGAARD, J ;

CANNON, B .

AMERICAN JOURNAL OF PHYSIOLOGY, 1988, 254 (02) :E155-E161

[10] DIFFERENTIATION OF OVINE BROWN ADIPOCYTE PRECURSOR CELLS IN A CHEMICALLY DEFINED SERUM-FREE MEDIUM - IMPORTANCE OF GLUCOCORTICOIDS AND AGE OF ANIMALS [J].

CASTEILLA, L ;

NOUGUES, J ;

REYNE, Y ;

RICQUIER, D .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1991, 198 (01) :195-199

← 1 2 3 4 5 →