A REFOCUSED AND OPTIMIZED HNCA - INCREASED SENSITIVITY AND RESOLUTION IN LARGE MACROMOLECULES

被引:69
作者
FARMER, BT
VENTERS, RA
SPICER, LD
WITTEKIND, MG
MULLER, L
机构
[1] BRISTOL MYERS SQUIBB,PHARMACEUT RES INST,PRINCETON,NJ 08543
[2] DUKE UNIV,MED CTR,DEPT BIOCHEM,DURHAM,NC 27710
关键词
3D NMR SPECTROSCOPY; REFOCUSED HNCA QUADRUPLE RESONANCE; MODIFIED INEPT SUBSEQUENCE; HNCA CORRELATION; SYNCHRONOUS H-1 BROAD-BAND DECOUPLING;
D O I
10.1007/BF01875530
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
A 3D optimized, refocused HNCA experiment is described. It is demonstrated to yield a dramatic increase in sensitivity when applied to [C-13, N-15]-labeled human carbonic anhydrase II, a 29-kDa protein. The reasons for the gain in sensitivity are discussed, and 3 distinct areas for further development are indicated.
引用
收藏
页码:195 / 202
页数:8
相关论文
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