IDENTIFICATION OF INTERACTING AMINO-ACIDS AT THE HISTONE 2A-2B BINDING-SITE

被引：42

作者：

DELANGE, RJ

WILLIAMS, LC

MARTINSON, HG

机构：

[1] UNIV CALIF LOS ANGELES, DEPT CHEM, DIV BIOCHEM, LOS ANGELES, CA 90024 USA

[2] UNIV CALIF LOS ANGELES, INST MOLEC BIOL, LOS ANGELES, CA 90024 USA

[3] UNIV CALIF LOS ANGELES, SCH MED, DEPT BIOL CHEM, LOS ANGELES, CA 90024 USA

来源：

BIOCHEMISTRY | 1979年 / 18卷 / 10期

关键词：

D O I：

10.1021/bi00577a014

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Histones 2A and 2B of calf thymus were cross-linked within intact nuclei by UV-irradiation. This procedure induces the formation of covalent cross-links between noncovalently interacting residues in the histones of native chromatin. Tryptic peptide and partial sequence analysis of the cross-linked product showed that the covalent linkage is between tyrosine-37, -40 or -42 of H2B and proline-26 of H2A. These residues constitute part of the hydrophobic H2A-H2B binding domain within the nucleosomes of native chromatin.

引用

页码：1942 / 1946

页数：5

共 13 条

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