STUDIES ON RIBONUCLEASES FROM PHYSARUM POLYCEPHALUM PURIFICATION AND CHARACTERIZATION OF SUBSTRATE SPECIFICITY

被引：25

作者：

HIRAMARU, M

UCHIDA, T

EGAMI, F

机构：

[1] Department of Biophysics and Biochemistry, Faculty of Science, University of Tokyo, Bunkyo-ku, Tokyo

来源：

JOURNAL OF BIOCHEMISTRY | 1969年 / 65卷 / 05期

关键词：

D O I：

10.1093/oxfordjournals.jbchem.a129068

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

1. Three RNases were isolated from Physarum polycephalum and designated RNase Pp-1, RNase Pp-2 and RNase Pp-3.2. They are thermostable.3. The approximate molecular weights estimated by gel filtration of RNases Pp-1 and Pp-2 are 40, 000 and that of RNase Pp-3 is 10,000.4. The optimum pH values of RNases Pp-1, Pp-2 and Pp-3 are pH6.7, pH 4.5 and pH5.5, respectively.5. RNase Pp-1 is a guanyloribonuclease (EC 2.7.7.26).6. RNase Pp-2 is a nonspecific RNase (EC 2.7.7.17).7. RNase Pp-3 may be classified as a nonspecific RNase (EC 2.7.7.17), but it attacks purine nucleotide bonds preferentially. © 1969 BY THE JOURNAL OF BIOCHEMISTRY.

引用

页码：693 / &

共 10 条

[1] STUDIES ON EXTRACELLULAR RIBONUCLEASES OF USTILAGO SPHAEROGENA - CHARACTERIZATION OF SUBSTRATE SPECIFICITY WITH SPECIAL REFERENCE TO PURINE-SPECIFIC RIBONUCLEASES [J].