SEQUENCE OF 1019 NUCLEOTIDES ENCOMPASSING ONE OF THE INVERTED REPEATS FROM THE YEAST 2 MU-M PLASMID

A sequence of 1019 nucleotides encompassing one of the 600 base inverted repeats and non-repeated flanking regions has been determined in the type A yeast 2 μm plasmid cloned in pMB9. Methods are described for applying the Maxarn-Gilbert sequencing procedure to DNA fragments labelled at the 3′-end using a T4-polymerase exchange/repair reaction and for sequencing 5′-end labelled fragments using dideoxy-nucleotides as chain terminators in the presence of E. coli DNA polymerase (nach Klenow). A notable feature of the sequence is its unusual content of symmetry elements. In one region of 140 nucleotides, 137 are involved in a complex arrangement of direct and inverted repeats linked by palindromic sequences. © 1979 Information Retrieval Limited.