QUANTITATIVE-DETERMINATION OF CATECHOLAMINES IN INDIVIDUAL BOVINE ADRENOMEDULLARY CELLS BY REVERSED-PHASE MICROCOLUMN LIQUID-CHROMATOGRAPHY WITH ELECTROCHEMICAL DETECTION

被引：80

作者：

COOPER, BR ^{[1
]}

JANKOWSKI, JA ^{[1
]}

LESZCZYSZYN, DJ ^{[1
]}

WIGHTMAN, RM ^{[1
]}

JORGENSON, JW ^{[1
]}

机构：

[1] UNIV N CAROLINA,DEPT CHEM,CHAPEL HILL,NC 27599

来源：

ANALYTICAL CHEMISTRY | 1992年 / 64卷 / 06期

关键词：

D O I：

10.1021/ac00030a022

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A microcolumn liquid chromatographic method for the determination of norepinephrine and epinephrine in single bovine adrenomedullary cells is described. A single cell is isolated from a culture plate, (3,4-dihydroxybenzyl)amine is added as an internal standard, and the cell is lysed with perchloric acid. After centrifugation, the supernatant is injected directly into a 42 or 50-mu-m inner diameter C18 reversed-phase column operated with electrochemical detection. Detection limits for norepinephrine and epinephrine were 46 and 75 amol, respectively. Of the 22 cells examined, 8 contained predominantly norepinephrine, 10 contained predominantly epinephrine, and at least 4 cells contained significant amounts of each. On average, a single cell contained approximately 150 fmol of catecholamine.

引用

页码：691 / 694

页数：4

共 23 条

[1]

BENEDECZKY A, 1964, NATURE, V204, P591

[2] VARIOUS CELL TYPES IN ADRENAL MEDULLA [J].